Department of Research and Development-Controlvet, Segurança Alimentar, S.A. - Zona Industrial de Tondela, ZIM II, L6, Tondela, Portugal.
J Appl Microbiol. 2009 Jun;106(6):1919-27. doi: 10.1111/j.1365-2672.2009.04145.x. Epub 2009 Feb 23.
To isolate and characterize bacteriophages, and to evaluate its lytic performance against avian pathogenic Escherichia coli (APEC) strains with high patterns of antibiotic resistance, in order to select phages for a therapeutic product to treat colibacillosis in chickens.
Bacteriophages were isolated from poultry sewage and tested against 148 O-serotyped APEC strains. The morphological characterization of the bacteriophages was made by transmission electronic microscopy (TEM) observations and the genetic comparison between bacteriophages DNA was performed by restriction fragment length polymorphism (RFLP) patterns. Results showed that 70.5% of the tested E. coli strains were sensitive to a combination of three of the five isolated phages, that seemed to be virulent and taxonomically belong to the Caudovirales order. Two of them look like 16-19, T4-like phages (Myoviridae) and the third is a T1-like phage and belongs to Syphoviridae family. All of them are genetically different.
It was possible to obtain a combination of three different lytic bacteriophages with broad lytic spectra against the most prevalent O-serotypes of APEC.
Data reported in this study, presents an in vitro well studied phage product to be used as antimicrobial agent to treat colibacillosis in poultry industry.
分离并鉴定噬菌体，并评估其对具有高度抗生素耐药模式的禽致病性大肠杆菌（APEC）菌株的裂解性能，以便选择噬菌体用于治疗鸡大肠杆菌病的治疗产品。
从家禽污水中分离出噬菌体，并对 148 株 O 血清型 APEC 菌株进行了测试。通过透射电子显微镜（TEM）观察对噬菌体进行了形态特征描述，并通过限制性片段长度多态性（RFLP）模式对噬菌体 DNA 进行了遗传比较。结果表明，测试的大肠杆菌菌株中有 70.5%对 5 种分离噬菌体中的 3 种组合敏感，这似乎是毒力噬菌体，且分类上属于长尾病毒目。其中两种看起来像 16-19、T4 样噬菌体（肌尾病毒科），第三种是 T1 样噬菌体，属于噬菌体型科。它们在遗传上都不同。
可以获得三种不同的溶菌噬菌体组合，对最常见的 APEC O 血清型具有广泛的溶菌谱。
本研究报告的数据提供了一种经过体外充分研究的噬菌体产品，可作为抗微生物剂用于治疗家禽养殖业中的大肠杆菌病。
