Mello-Neto Julio, Rondó Patricia H C, Oshiiwa Marie, Morgano Marcelo A, Zacari Cristiane Z, Domingues Sandra
Department of Histology, State University of Londrina, Londrina, Paraná, Brazil.
Clin Nutr. 2009 Apr;28(2):178-81. doi: 10.1016/j.clnu.2009.02.002. Epub 2009 Feb 26.
BACKGROUND & AIMS: This study evaluated the relationship between vitamin A concentration in maternal milk and the characteristics of the donors of a Brazilian human milk bank.
A total of 136 donors were selected in 2003-2004 for micronutrient determinations in breast milk and blood, anthropometric measurements and investigation of obstetric, socioeconomic-demographic factors, and life style. Maternal serum/milk samples were obtained for vitamin A, iron, copper, and zinc determinations. Vitamin A concentrations in breast milk and blood were assessed by high-performance liquid chromatography. Copper, zinc and iron concentrations in breast milk, and copper and zinc concentrations in blood were detected by atomic emission spectrophotometry. Serum ceruloplasmin and serum iron were determined, respectively, by nephelometry and colorimetry. A linear regression model assessed the associations between milk concentrations of vitamin A and maternal factors.
Vitamin A in milk presented positive associations with iron in milk (p<0.001), serum retinol (p=0.03), maternal work (p=0.02), maternal age (p=0.02), and oral contraceptive use (p=0.01), and a negative association with % body fat (p=0.01) (R(2)=0.47).
These results suggest that some nutritional, obstetric, and socioeconomic-demographic factors may have an effect on mature breast milk concentrations of vitamin A in apparently healthy Brazilian mothers.
本研究评估了母乳中维生素A浓度与巴西一家母乳库捐赠者特征之间的关系。
2003年至2004年共选取了136名捐赠者，对其母乳和血液中的微量营养素进行测定，进行人体测量，并调查产科、社会经济人口因素及生活方式。采集母亲的血清/母乳样本用于测定维生素A、铁、铜和锌。母乳和血液中的维生素A浓度通过高效液相色谱法评估。母乳中的铜、锌和铁浓度以及血液中的铜和锌浓度通过原子发射分光光度法检测。血清铜蓝蛋白和血清铁分别通过散射比浊法和比色法测定。采用线性回归模型评估母乳中维生素A浓度与母亲因素之间的关联。
母乳中的维生素A与母乳中的铁（p<0.001）、血清视黄醇（p=0.03）、母亲工作（p=0.02）、母亲年龄（p=0.02）以及口服避孕药的使用（p=0.01）呈正相关，与体脂百分比呈负相关（p=0.01）（R²=0.47）。
这些结果表明，一些营养、产科和社会经济人口因素可能会对明显健康的巴西母亲成熟母乳中维生素A的浓度产生影响。
