生物组织的电阻抗谱研究
Electrical Impedance Spectroscopy Study of Biological Tissues.
作者信息
Dean D A, Ramanathan T, Machado D, Sundararajan R
机构信息
Division of Pulmonary & Critical Care, Northwestern University Medical School, Chicago, IL-60611, e-mail:
出版信息
J Electrostat. 2008 Mar;66(3-4):165-177. doi: 10.1016/j.elstat.2007.11.005.
The objective of this study was to investigate the electrical impedance properties of rat lung and other tissues ex vivo using Electrical Impedance Spectroscopy. Rat lungs (both electroporated and naïve (untreated)), and mesenteric vessels (naïve) were harvested from male Sprague-Dawley rats; their electrical impedance were measured using a Solartron 1290 impedance analyzer. Mouse lung and heart samples (naïve) were also studied. The resistance (Real Z, ohm) and the reactance (Im Z, negative ohm)) magnitudes and hence the Cole-Cole (Real Z versus Im Z) plots are different for the electroporated lung and the naive lung. The results confirm the close relationship between the structure and the functional characteristic. These also vary for the different biological tissues studied. The impedance values were higher at low frequencies compared to those at high frequencies. This study is of practical interest for biological applications of electrical pulses, such as electroporation, whose efficacy depends on cell type and its electrical impedance characteristics.
本研究的目的是使用电阻抗光谱法体外研究大鼠肺及其他组织的电阻抗特性。从雄性Sprague-Dawley大鼠身上获取大鼠肺（电穿孔处理的和未处理的）以及肠系膜血管（未处理的）；使用Solartron 1290阻抗分析仪测量它们的电阻抗。还对小鼠肺和心脏样本（未处理的）进行了研究。电穿孔处理的肺和未处理的肺的电阻（实部Z，欧姆）和电抗（虚部Z，负欧姆）大小以及因此得到的科尔-科尔图（实部Z对虚部Z）是不同的。结果证实了结构与功能特性之间的密切关系。对于所研究的不同生物组织，这些关系也有所不同。与高频时相比，低频时的阻抗值更高。本研究对于电阻抗在生物应用（如电穿孔）中的实际应用具有重要意义，电穿孔的效果取决于细胞类型及其电阻抗特性。
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