Snyder Daniel W, Opperman Charles H, Bird David McK
Center for the Biology of Nematode Parasitism, NC State University, Raleigh, NC 27695.
J Nematol. 2006 Jun;38(2):192-4.
A method for producing mass quantities of Meloidogyne incognita males free from other developmental stages was developed. Host plants were grown hydroponically to facilitate nematode harvest. Pruning stress was shown to cause a higher percentage of juveniles to develop as males vs. a no-stress control. Application of pruning stress in the first 48 hr post-inoculation was also shown to be more effective at driving male development than at later times.
开发了一种生产大量不含其他发育阶段的南方根结线虫雄虫的方法。寄主植物采用水培方式种植以利于线虫收获。结果表明，与无胁迫对照相比，修剪胁迫会导致更高比例的幼虫发育为雄虫。接种后前48小时施加修剪胁迫在促进雄虫发育方面也比后期更有效。
