Spence Kenneth O, Lewis Edwin E, Perry Roland N
Department of Nematology, UC Davis, Davis, CA 95616. Plant Pathology and Microbiology Department, Rothamsted Research, Harpenden, Herts. AL5 2JQ, UK.
J Nematol. 2008 Jun;40(2):93-8.
Directly viewing soil-dwelling entomopathogenic and plant-parasitic nematodes in situ is difficult, if not impossible. As a result, researchers have developed a diverse array of bioassays which assess nematode behavioral traits within arenas designed to simulate various aspects of the natural habitat. However, reliably rendering what we can see in the laboratory into accurate predictions of how nematodes achieve their objectives in the field is challenging. In the current review, we systemically assessed the goals and attributes of several of the assays most commonly used to investigate nematode host finding and host invasion behavior. By illuminating the relative strengths and limitations of each assay, we hope to improve our ability to develop meaningful predictions for the field.
直接在原位观察土壤中的昆虫病原线虫和植物寄生线虫即便不是完全不可能，也是很困难的。因此，研究人员开发了各种各样的生物测定方法，这些方法在旨在模拟自然栖息地各个方面的实验场地中评估线虫的行为特征。然而，要将我们在实验室中看到的情况可靠地转化为对线虫在野外实现其目标方式的准确预测，是具有挑战性的。在当前的综述中，我们系统地评估了几种最常用于研究线虫寄主寻找和寄主入侵行为的测定方法的目标和特性。通过阐明每种测定方法的相对优势和局限性，我们希望提高我们为野外情况做出有意义预测的能力。
