Yao Yanli, Bai Xiaoyun, Shiu Kwok-Keung
Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
Nanomaterials (Basel). 2012 Nov 27;2(4):428-444. doi: 10.3390/nano2040428.
A simple method has been developed for the spontaneous deposition of Prussian blue (PB) particles from a solution containing only ferricyanide ions onto conducting substrates such as indium tin oxide glass, glassy carbon disk and carbon nanotube (CNT) materials. Formation of PB deposits was confirmed by ultraviolet-visible absorption spectrometry and electrochemical techniques. The surface morphology of the PB particles deposited on the substrates was examined by atomic force microscopy and scanning electron microscopy. CNT/PB composite modified glassy carbon electrodes exhibited an electrocatalytic property for hydrogen peroxide reduction. These modified electrodes exhibited a high sensitivity for electrocatalytic reduction of hydrogen peroxide at -0.05 V ( Ag|AgCl), probably due to the synergistic effect of CNT with PB. Then, CNT/PB modified electrodes were further developed as amperometric glucose biosensors. These biosensors offered a linear response to glucose concentration from 0.1 to 0.9 mM with good selectivity, high sensitivity of 0.102 A M¹ cm and short response time (within 2 s) at a negative operation potential of -0.05 V ( Ag|AgCl). The detection limit was estimated to be 0.01 mM at a signal-to-noise ratio of 3.
已开发出一种简单方法，可使普鲁士蓝（PB）颗粒从仅含氰铁离子的溶液自发沉积到诸如氧化铟锡玻璃、玻碳圆盘和碳纳米管（CNT）材料等导电基底上。通过紫外可见吸收光谱法和电化学技术确认了PB沉积物的形成。用原子力显微镜和扫描电子显微镜检查了沉积在基底上的PB颗粒的表面形态。CNT/PB复合修饰玻碳电极对过氧化氢还原表现出电催化性能。这些修饰电极在-0.05 V（Ag|AgCl）下对过氧化氢的电催化还原表现出高灵敏度，这可能归因于CNT与PB的协同效应。然后，将CNT/PB修饰电极进一步开发为电流型葡萄糖生物传感器。这些生物传感器在-0.05 V（Ag|AgCl）的负操作电位下，对葡萄糖浓度在0.1至0.9 mM范围内呈现线性响应，具有良好的选择性、0.102 A M⁻¹ cm的高灵敏度和短响应时间（2 s内）。在信噪比为3时，检测限估计为0.01 mM。
