Locsei Zoltan, Racz Karoly, Patocs Attila, Kovacs Gabor L, Toldy Erzsebet
1st Department of Medicine Markusovszky Teaching Hospital of County Vas, Szombathely, Hungary.
Clin Chim Acta. 2009 Apr;402(1-2):203-5. doi: 10.1016/j.cca.2009.01.013.
Because no systematic study on the comparison of the stability of plasma renin activity (PRA) and renin concentration (REN) under different preanalytical conditions has been reported, we examined the impact of several sampling and storage conditions.
Blood samples were collected into prechilled tubes and into tubes at room temperature and processed within 30 min or kept at room temperature or at 0-5 degrees C for 2 h. The stability of PRA and REN after storage at - 20 degrees C was also analysed. PRA and REN were determined with radioimmunoassay (DiaSorin) and chemiluminescent immunometric method (LIAISON, DiaSorin), respectively.
When blood samples were kept at room temperature for 2 h, the PRA and REN decreased significantly (p < 0.001), but both remained unchanged if samples were placed at 0-5 degrees C for the same time period. The REN was significantly higher in samples collected in prechilled tubes and refrigerated within 30 min at 0-5 degrees C compared to samples processed at room temperature (p < 0.05). During storage at -20 degrees C for 7 weeks the PRA decreased by 31.9 +/- 4.7%, while the change in REN remained below 5%.
Different sampling and storage conditions differentially influence the PRA and REN, which makes it necessary to apply well-defined preanalytical procedures for PRA and REN measurements.
由于尚无关于不同分析前条件下血浆肾素活性（PRA）和肾素浓度（REN）稳定性比较的系统研究报道，我们研究了几种采样和储存条件的影响。
将血样采集到预冷管和室温管中，在30分钟内进行处理，或在室温或0 - 5摄氏度下保存2小时。还分析了在-20摄氏度储存后PRA和REN的稳定性。分别采用放射免疫分析法（DiaSorin）和化学发光免疫分析法（LIAISON，DiaSorin）测定PRA和REN。
当血样在室温下保存2小时时，PRA和REN显著下降（p < 0.001），但如果在相同时间段内将样品置于0 - 5摄氏度，则两者均保持不变。与在室温下处理的样品相比，在预冷管中采集并在0 - 5摄氏度下30分钟内冷藏的样品中REN显著更高（p < 0.05）。在-20摄氏度下储存7周期间，PRA下降了31.9 +/- 4.7%，而REN的变化保持在5%以下。
不同的采样和储存条件对PRA和REN有不同影响，这使得有必要对PRA和REN测量采用明确的分析前程序。
