Lu Xuebin, Zhang Yimin, Angelidaki Irini
Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Bioresour Technol. 2009 Jun;100(12):3048-53. doi: 10.1016/j.biortech.2009.01.008. Epub 2009 Mar 5.
A central composite design of response surface method was used to optimize H(2)SO(4)-catalyzed hydrothermal pretreatment of rapeseed straw, in respect to acid concentration (0.5-2%), treatment time (5-20 min) and solid content (10-20%) at 180 degrees C. Enzymatic hydrolysis and fermentation were also measured to evaluate the optimal pretreatment conditions for maximizing ethanol production. The results showed that acid concentration and treatment time were more significant than solid content for optimization of xylose release and cellulose recovery. Pretreatment with 1% sulfuric acid and 20% solid content for 10 min at 180 degrees C was found to be the most optimal condition for pretreatment of rapeseed straw for ethanol production. After pretreatment at the optimal condition and enzymatic hydrolysis, 75.12% total xylan and 63.17% total glucan were converted to xylose and glucose, respectively. Finally, 66.79% of theoretical ethanol yielded after fermentation.
采用响应面法的中心复合设计，在180℃下，针对硫酸浓度（0.5 - 2%）、处理时间（5 - 20分钟）和固含量（10 - 20%）对油菜秸秆进行硫酸催化水热预处理优化。还通过测定酶水解和发酵情况来评估使乙醇产量最大化的最佳预处理条件。结果表明，在优化木糖释放和纤维素回收率方面，酸浓度和处理时间比固含量更显著。发现180℃下用1%硫酸和20%固含量处理10分钟是油菜秸秆乙醇生产预处理的最佳条件。在最佳条件下预处理并酶水解后，总木聚糖的75.12%和总葡聚糖的63.17%分别转化为木糖和葡萄糖。最终，发酵后乙醇产量达到理论产量的66.79%。
