Rashidi Hassan, Sottile Virginie
Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), School of Clinical Sciences, The University of Nottingham, Nottingham, UK.
Bioessays. 2009 Apr;31(4):459-65. doi: 10.1002/bies.200800168.
Animal models play a crucial role in fundamental and medical research. Progress in the fields of drug discovery, regenerative medicine and cancer research among others are heavily dependent on in vivo models to validate in vitro observations, and develop new therapeutic approaches. However, conventional rodent and large animal experiments face ethical, practical and technical issues that limit their usage. The chick embryo represents an accessible and economical in vivo model, which has long been used in developmental biology, gene expression analysis and loss/gain of function experiments. It is also an established model for tissue/cell transplantation, and because of its lack of immune system in early development, the chick embryo is increasingly recognised as a model of choice for mammalian biology with new applications for stem cell and cancer research. Here, we review novel applications of the chick embryo model, and discuss future developments of this in vivo model for biomedical research.
动物模型在基础研究和医学研究中发挥着至关重要的作用。药物发现、再生医学和癌症研究等领域的进展在很大程度上依赖于体内模型来验证体外观察结果，并开发新的治疗方法。然而，传统的啮齿动物和大型动物实验面临着伦理、实际和技术问题，这些问题限制了它们的使用。鸡胚是一种易于获取且经济的体内模型，长期以来一直用于发育生物学、基因表达分析以及功能丧失/获得实验。它也是组织/细胞移植的成熟模型，并且由于其在早期发育中缺乏免疫系统，鸡胚越来越被认为是哺乳动物生物学的首选模型，在干细胞和癌症研究中有新的应用。在此，我们综述鸡胚模型的新应用，并讨论这种体内模型在生物医学研究中的未来发展。
