Phani Victor, Shivakumara Tagginahalli N, Davies Keith G, Rao Uma
Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, India.
School of Life and Medical Sciences, University of Hertfordshire, Hatfield, United Kingdom.
Front Microbiol. 2017 Nov 1;8:2122. doi: 10.3389/fmicb.2017.02122. eCollection 2017.
Root-knot nematode (RKN) is an economically important pest of crops. , is a nematode hyperparasitic bacterium capable of suppressing the reproduction of RKN and thereby useful for its management. Secreted fatty acid and retinol-binding proteins are unique in nematodes and are engaged in nutrient acquisition, development and reproduction; they are also a component of the nematode cuticle and thought to be involved in the interface between hosts and parasites. Attachment of endospores to the cuticle of second stage juveniles of RKN is the primary step of infection and several factors have been identified to facilitate attachment. In this study, the full length of (573 bp) was cloned from and characterized. Analysis revealed that the was rich in α-helix structure, contained a predicted consensus casein kinase II phosphorylation site and a glycosylation site. Quantitative PCR showed the highest expression in the fourth stage juveniles and hybridization revealed the presence of mRNA in the hypodermis below the cuticle. Single copy insertion pattern of in genome was detected by Southern blotting. Knockdown of showed significantly increased attachment of endospores on juvenile cuticle surface and also affected host finding, root infection and nematode fecundity.
根结线虫（RKN）是一种对农作物具有重要经济影响的害虫。[具体细菌名称未给出]是一种线虫超寄生细菌，能够抑制根结线虫的繁殖，因此对其防治具有重要作用。分泌的脂肪酸和视黄醇结合蛋白在 nematodes 中是独特的，参与营养获取、发育和繁殖；它们也是线虫角质层的组成部分，被认为参与宿主与寄生虫之间的界面。内生孢子附着在根结线虫第二阶段幼虫的角质层上是感染的第一步，并且已经确定了几个促进附着的因素。在本研究中，从[具体来源未给出]克隆了[具体基因名称未给出]（573 bp）的全长并进行了表征。分析表明，[具体基因名称未给出]富含α-螺旋结构，包含一个预测的共有酪蛋白激酶II磷酸化位点和一个糖基化位点。定量PCR显示在第四阶段幼虫中表达最高，并且[具体杂交方法未给出]杂交揭示了在角质层下方的皮下组织中存在[具体基因名称未给出]mRNA。通过Southern印迹检测到[具体基因名称未给出]在[具体基因组未给出]基因组中的单拷贝插入模式。敲低[具体基因名称未给出]显示[具体细菌名称未给出]内生孢子在幼虫角质层表面的附着显著增加，并且还影响宿主寻找、根部感染和线虫繁殖力。
