Saito Takashi, Iinuma Yoshitsugu, Takakura Shunji, Nagao Miki, Matsushima Aki, Shirano Michinori, Ichiyama Satoshi
Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
J Infect Chemother. 2009 Feb;15(1):49-53. doi: 10.1007/s10156-008-0664-6. Epub 2009 Mar 12.
This study examined the effects on patients with bacteremia of delaying the insertion of a blood culture bottle into an automated, continuously monitoring blood culture system. We investigated the time taken from the collection of blood samples (collection) to the insertion of blood culture bottles inoculated with blood samples into the instrument (insertion), and compared the mean detection time from collection to a positive signal from the instrument with the time between collection and insertion. The study was conducted from January 2003 to December 2004 at Kyoto University Hospital. Insertion into the system on the day of blood sample collection was defined as same-day insertion, and insertion on a different day to collection was defined as delayed insertion. The 7394 aerobic and anaerobic blood culture bottle sets obtained during the study period included 4361 sets with same-day insertion and 3033 sets with delayed insertion. For same-day insertion, 458 microorganisms were isolated from 432 positive sets in which at least one blood culture bottle was positive. For delayed insertion, 405 microorganisms were isolated from 379 positive sets in which at least one blood culture bottle was positive. The mean detection time for all microorganisms was significantly earlier for same-day insertion than for delayed insertion (28.3 h vs. 45.0 h, respectively, P < 0.0001). Delays from collection to insertion affect the time from collection to the detection of microorganisms.
本研究探讨了延迟将血培养瓶插入自动连续监测血培养系统对菌血症患者的影响。我们调查了从采集血样（采集）到将接种血样的血培养瓶插入仪器（插入）所需的时间，并将从采集到仪器发出阳性信号的平均检测时间与采集和插入之间的时间进行了比较。该研究于2003年1月至2004年12月在京都大学医院进行。在血样采集当天插入系统被定义为同日插入，在与采集不同的日期插入被定义为延迟插入。研究期间获得的7394套需氧和厌氧血培养瓶中，有4361套为同日插入，3033套为延迟插入。对于同日插入，从432套至少一个血培养瓶呈阳性的阳性培养物中分离出458种微生物。对于延迟插入，从379套至少一个血培养瓶呈阳性的阳性培养物中分离出405种微生物。所有微生物的平均检测时间同日插入明显早于延迟插入（分别为28.3小时和45.0小时，P<0.0001）。从采集到插入的延迟会影响从采集到检测出微生物的时间。
