Qin Ling, Li Bogang, Guan Jiafa, Zhang Guolin
Chengdu Institute of Biology, Chinese Academy of Sciences, People's Republic of China.
Nat Prod Res. 2009;23(4):309-18. doi: 10.1080/14786410801972813.
An agar plate method was established to screen synergistic antibacterial agents other than beta-lactamase inhibitors. By using this method, a strain Aspergillus sp136 was selected for further studies. From the metabolites of this strain, a synergistic antibacterial compound was isolated by bioautographic TLC assay-guided fractionation and identified as helvolic acid. The synergistic effect of helvolic acid to penicillin was about 3 times that of clavulanic acid to penicillin in agar diffusion assay on Bacillus cereus. In checkerboard studies, helvolic acid exhibited synergistic effects with erythromycin on all tested multi-drug resistant Staphylococcus aureus and with penicillin and tetracycline on some multi-drug resistant S. aureus. A pattern of enhanced killing was also found in time-kill studies on multi-drug resistant S. aureus.
建立了一种琼脂平板法来筛选除β-内酰胺酶抑制剂以外的协同抗菌剂。通过使用该方法，选择了一株曲霉属sp136菌株进行进一步研究。从该菌株的代谢产物中，通过生物自显影薄层色谱分析引导的分级分离法分离出一种协同抗菌化合物，并鉴定为浅黄霉素。在蜡样芽孢杆菌的琼脂扩散试验中，浅黄霉素对青霉素的协同作用约为克拉维酸对青霉素协同作用的3倍。在棋盘法研究中，浅黄霉素对所有测试的耐多药金黄色葡萄球菌与红霉素表现出协同作用，对一些耐多药金黄色葡萄球菌与青霉素和四环素表现出协同作用。在对耐多药金黄色葡萄球菌的时间杀菌研究中也发现了增强杀灭的模式。
