Moglia Andrea, Comino Cinzia, Portis Ezio, Acquadro Alberto, De Vos Ric C H, Beekwilder Jules, Lanteri Sergio
DiVaPRA, Plant Genetics and Breeding, University of Torino, via L. da Vinci 44, 10095, Grugliasco (TO), Italy.
Plant Cell Rep. 2009 Jun;28(6):963-74. doi: 10.1007/s00299-009-0695-1. Epub 2009 Mar 20.
Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl ester 3'-hydroxylase (CYP98A49), which is involved in both chlorogenic acid and lignin biosynthesis. Phylogenetic analyses demonstrated that this gene belongs to the CYP98 family. CYP98A49 was also heterologously expressed in yeast, in order to perform an enzymatic assay with p-coumaroylshikimate and p-coumaroylquinate as substrates. Real Time quantitative PCR analysis revealed that CYP98A49 expression is induced upon exposure to UV-C radiation. A single nucleotide polymorphism in the CYP98A49 gene sequence of two globe artichoke varieties used for genetic mapping allowed the localization of this gene to linkage group 10 within the previously developed maps.
朝鲜蓟是酚类化合物的天然来源，其中二咖啡酰奎宁酸及其生物合成前体绿原酸（5-咖啡酰奎宁酸）是主要分子。我们报道了朝鲜蓟对香豆酰酯3'-羟化酶（CYP98A49）全长cDNA和启动子的分离与鉴定，该酶参与绿原酸和木质素的生物合成。系统发育分析表明该基因属于CYP98家族。CYP98A49也在酵母中进行了异源表达，以便以对香豆酰莽草酸酯和对香豆酰奎尼酸为底物进行酶活性测定。实时定量PCR分析表明，暴露于UV-C辐射后，CYP98A49的表达被诱导。用于遗传图谱构建的两个朝鲜蓟品种的CYP98A49基因序列中的一个单核苷酸多态性，使得该基因能够定位到先前构建的图谱中的第10连锁群上。
