Gadiou S, Safárová D, Navrátil M
Palacký University in Olomouc, Holice, Czech Republic.
Acta Virol. 2009;53(1):53-6.
Single-strand conformation polymorphism (SSCP) and low-stringency single specific primer (LSSP)-PCR were assessed for suitability and reliability in genotyping of Plum pox virus (PPV) isolates. Examined PPV isolates included 16 PPV-D, 12 PPV-M, and 14 PPV-Rec isolates collected in Czech Republic. The analysis was performed on the helper component protease (HC-Pro) region of the PPV genome. SSCP and LSSP-PCR allowed the differentiation of PPV strain, but SSCP was not able to distinguish isolates within the same strain. The individual genotyping of each PPV isolate was obtained by LSSP-PCR. Nevertheless, both SSCP and LSSP-PCR techniques are suitable for preliminary screening of genetic variability of plant RNA viruses.
对单链构象多态性（SSCP）和低严谨度单特异性引物（LSSP）-PCR在李痘病毒（PPV）分离株基因分型中的适用性和可靠性进行了评估。所检测的PPV分离株包括在捷克共和国收集的16株PPV-D、12株PPV-M和14株PPV-Rec分离株。分析针对PPV基因组的辅助成分蛋白酶（HC-Pro）区域进行。SSCP和LSSP-PCR能够区分PPV毒株，但SSCP无法区分同一毒株内的分离株。通过LSSP-PCR获得了每个PPV分离株的个体基因分型。然而，SSCP和LSSP-PCR技术都适用于植物RNA病毒遗传变异性的初步筛选。
