College of Chemistry and Biology, Shenyang Normal University, Shenyang, Liaoning, 110034, China,
J Am Soc Mass Spectrom. 2013 Oct;24(10):1612-5. doi: 10.1007/s13361-013-0697-7. Epub 2013 Jul 27.
This study presents a novel direct analysis strategy for rapid mass spectrometric profiling of biochemicals in real-world samples via a direct sampling probe (DSP) without sample pretreatments. Chemical modification is applied to a disposable stainless steel acupuncture needle to enhance its surface area and hydrophilicity. After insertion into real-world samples, biofluid can be attached on the DSP surface. With the presence of a high DC voltage and solvent vapor condensing on the tip of the DSP, analyte can be dissolved and electrosprayed. The simplicity in design, versatility in application aspects, and other advantages such as low cost and disposability make this new method a competitive tool for direct analysis of real-world samples.
本研究提出了一种新颖的直接分析策略，通过直接采样探针（DSP）在无需样品预处理的情况下，快速进行质谱分析对真实样品中的生物化学物质进行分析。采用化学修饰的方法对一次性不锈钢针灸针进行处理，以增强其表面积和亲水性。将其插入真实样品后，生物体液可附着在 DSP 表面。在高直流电压的存在下，溶剂蒸汽在 DSP 尖端冷凝，分析物可溶解并进行电喷雾。该方法设计简单、应用范围广泛，具有低成本和一次性等优点，是直接分析真实样品的一种有竞争力的工具。
