Köhnke Rickard, Lindbo Agnes, Larsson Therese, Lindqvist Andreas, Rayner Marilyn, Emek Sinan C, Albertsson Per-Ake, Rehfeld Jens F, Landin-Olsson Mona, Erlanson-Albertsson Charlotte
Department of Experimental Medical Science, Appetite Control Unit, BMC B11, Lund University, Lund, Sweden.
Scand J Gastroenterol. 2009;44(6):712-9. doi: 10.1080/00365520902803499.
The effects of a promising new appetite suppressor named "thylakoids" (membrane proteins derived from spinach leaves) were examined in a single meal in man. Thylakoids inhibit the lipase/colipase hydrolysis of triacylglycerols in vitro and suppress food intake, decrease body-weight gain and raise the satiety hormone cholecystokinin (CCK) in rats, but their effects in man remain unclear. The aim of this study was to investigate whether thylakoids, when added to a test meal, affect appetite regulation and blood parameters in healthy individuals.
In an intervention crossover study, healthy individuals of normal weight (n=11) were offered a high-fat meal with and without the addition of thylakoids. Blood samples were taken 0 (prior to meal), 30, 60, 120, 180, 240, 300 and 360 min after the start of the meal. Blood samples were analysed for satiety and hunger hormones (CCK, leptin and ghrelin), insulin and blood metabolites (glucose and free fatty acids).
The CCK level increased, in particular between the 120 min time-point and onwards, the ghrelin level was reduced at 120 min and leptin level increased at 360 min after intake of the thylakoid-enriched meal. The insulin level was reduced, whereas glucose concentrations were unchanged. Free fatty acids were reduced between time-point 120 min and onwards after the thylakoid meal.
The addition of thylakoids to energy-dense food promotes satiety signals and reduces insulin response during a single meal in man.
在人体单次进餐过程中研究一种名为“类囊体”（从菠菜叶中提取的膜蛋白）的新型有前景的食欲抑制剂的作用。类囊体在体外可抑制三酰甘油的脂肪酶/共脂肪酶水解，并能抑制大鼠的食物摄入、减少体重增加以及提高饱腹感激素胆囊收缩素（CCK），但其在人体中的作用尚不清楚。本研究的目的是调查在健康个体中，将类囊体添加到测试餐中是否会影响食欲调节和血液参数。
在一项干预性交叉研究中，为正常体重的健康个体（n = 11）提供添加和不添加类囊体的高脂餐。在进餐开始后的0（进餐前）、30、60、120、180、240、300和360分钟采集血样。分析血样中的饱腹感和饥饿激素（CCK、瘦素和胃饥饿素）、胰岛素以及血液代谢物（葡萄糖和游离脂肪酸）。
摄入富含类囊体的餐后，CCK水平升高，尤其是在120分钟及之后的时间点；胃饥饿素水平在120分钟时降低，瘦素水平在360分钟时升高。胰岛素水平降低，而葡萄糖浓度未变。类囊体餐后，游离脂肪酸在120分钟及之后的时间点降低。
在人体单次进餐过程中，向能量密集型食物中添加类囊体可促进饱腹感信号并降低胰岛素反应。
