Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Attar-neishabouri Ave, Golgasht St, Tabriz, 5165665931, Iran.
Nutr J. 2020 Aug 11;19(1):82. doi: 10.1186/s12937-020-00601-4.
There is a promising outlook regarding the potential effect of spinach-derived thylakoids in the management of obesity and its associated metabolic disturbances. This research aimed to evaluate the effects of spinach-derived thylakoids supplementation combined with a calorie-restricted diet on anthropometric and metabolic profiles in obese women with the polycystic ovary syndrome (PCOS).
In a 12-week double-blind placebo-controlled randomized clinical trial, 48 females with obesity and PCOS were randomly allocated into either intervention (5 g/day thylakoid) or placebo (5 g/day cornstarch) groups along with calorie-restricted diets. Anthropometric measures, physical activity levels, dietary intakes, insulin resistance markers, as well as serum levels of insulin, fasting blood glucose (FBG), non-esterified fatty acids (NEFA), and sex hormones including dehydroepiandrosterone sulfate (DHEAS), follicle-stimulating hormone (FSH), luteinizing hormone (LH), sex hormone-binding globulin (SHBG), and free androgen index (FAI) were evaluated pre-and post-intervention.
After the 12-week intervention, there were significant decreases in weight (- 6.97 ± 0.52 vs. -3.19 ± 0.72 kg; P < 0.001), waist circumference (- 7.78 ± 2.50 vs. -3.73 ± 1.40 cm; P < 0.001), fat mass (- 5.19 ± 0.53 vs. -1.36 ± 0.39 kg; P < 0.001), and insulin levels (- 5.40 ± 1.86 vs. -1.19 ± 0.85 μU/mL; P < 0.001) in the spinach-derived thylakoid group compared to the placebo group. Furthermore, insulin resistance markers and serum levels of testosterone decreased significantly in the thylakoid group compared to the placebo group (P < 0.05). The changes in other parameters did not show significant differences between the two groups.
Spinach-derived thylakoid supplementation resulted in more favorable improvements in anthropometric indices and insulin sensitivity compared to the calorie restriction alone.
The study was approved by the Ethics Committee of Research Vice-chancellor of Tabriz University of Medical Sciences, Tabriz, Iran, and was registered in the Iranian Registry of Clinical Trials (registration ID: IRCT20140907019082N9 ).
菠菜衍生的类囊体在肥胖症及其相关代谢紊乱的管理中具有潜在的积极作用。本研究旨在评估菠菜衍生的类囊体补充剂与热量限制饮食联合应用对多囊卵巢综合征（PCOS）肥胖女性的人体测量和代谢特征的影响。
在一项为期 12 周的双盲安慰剂对照随机临床试验中，将 48 名肥胖和 PCOS 女性随机分为干预组（每天 5g 类囊体）和安慰剂组（每天 5g 玉米淀粉），同时进行热量限制饮食。在干预前和干预后评估人体测量指标、身体活动水平、饮食摄入量、胰岛素抵抗标志物以及血清胰岛素、空腹血糖（FBG）、非酯化脂肪酸（NEFA）和性激素，包括脱氢表雄酮硫酸盐（DHEAS）、卵泡刺激素（FSH）、黄体生成素（LH）、性激素结合球蛋白（SHBG）和游离雄激素指数（FAI）。
12 周干预后，干预组体重（-6.97±0.52 与-3.19±0.72kg；P<0.001）、腰围（-7.78±2.50 与-3.73±1.40cm；P<0.001）、脂肪量（-5.19±0.53 与-1.36±0.39kg；P<0.001）和胰岛素水平（-5.40±1.86 与-1.19±0.85μU/mL；P<0.001）均显著下降，而安慰剂组无明显变化。此外，与安慰剂组相比，类囊体组的胰岛素抵抗标志物和血清睾酮水平也显著下降（P<0.05）。两组其他参数的变化无统计学差异。
与单纯热量限制相比，菠菜衍生的类囊体补充剂可更显著地改善人体测量指标和胰岛素敏感性。
该研究得到了伊朗大不里士塔比里斯医科大学研究副校长伦理委员会的批准，并在伊朗临床试验注册处注册（注册号：IRCT20140907019082N9）。
