Li Min-Hui, Huang Kun-Lun, Wu Shu-Yu, Chen Chien-Wen, Yan Horng-Chin, Hsu Kang, Hsu Ching-Wang, Tsai Shih-Hung, Chu Shi-Jye
Institute of Aerospace Medicine, National Defense Medical Center, Taipei, Taiwan.
Br J Pharmacol. 2009 May;157(2):244-51. doi: 10.1111/j.1476-5381.2009.00139.x. Epub 2009 Mar 20.
Baicalin has been reported to have anti-inflammatory effects and protect against various tissue injuries. However, the effect of baicalin on air embolism-induced acute lung injury has not been tested yet.
Acute lung injury was induced by infusion of air at a rate of 0.25 mL.min(-1) for 1 min into the pulmonary artery of rat isolated lungs. At the end of the experiment, samples were collected for assessment of lung injury, biochemical analysis and histology. Different doses of baicalin (1, 2 and 4 mg.kg(-1)) were given into the perfusate before air infusion.
Air embolism elicited a significant increase in microvascular permeability (K(f)), lung weight gain, wet/dry weight ratio, pulmonary artery pressure and protein concentration in the bronchoalveolar lavage fluid. Levels of the cytokines, tumour necrosis factor alpha and cytokine-induced neutrophil chemoattractant-1 in perfusate, and malondialdehyde levels and myeloperoxidase activities in lung tissue were also significantly increased. In addition, histological examination showed increased neutrophil infiltration in lung tissues. Furthermore, nuclear factor-kappaB activity and degradation of IkappaB-alpha were significantly increased in lungs. Pretreatment of the lungs with baicalin (4 mg.kg(-1)) showed a statistically significant difference in all of the assessed parameters, except for alteration in the pulmonary artery pressure.
Our study suggests that baicalin attenuated air embolism-induced acute lung injury and may be considered a useful adjunct drug therapy in this clinical condition.
据报道，黄芩苷具有抗炎作用，并能预防各种组织损伤。然而，黄芩苷对空气栓塞所致急性肺损伤的影响尚未得到验证。
通过以0.25 mL·min⁻¹的速率向大鼠离体肺的肺动脉内注入空气1分钟来诱导急性肺损伤。实验结束时，收集样本以评估肺损伤、进行生化分析和组织学检查。在注入空气前，将不同剂量的黄芩苷（1、2和4 mg·kg⁻¹）加入灌注液中。
空气栓塞导致微血管通透性（K(f)）显著增加、肺重量增加、湿/干重比增加、肺动脉压升高以及支气管肺泡灌洗液中蛋白质浓度升高。灌注液中细胞因子肿瘤坏死因子α和细胞因子诱导的中性粒细胞趋化因子-1的水平，以及肺组织中丙二醛水平和髓过氧化物酶活性也显著增加。此外，组织学检查显示肺组织中中性粒细胞浸润增加。此外，肺组织中核因子-κB活性和IκB-α降解显著增加。用黄芩苷（4 mg·kg⁻¹）预处理肺组织后，除肺动脉压改变外，所有评估参数均有统计学显著差异。
我们的研究表明，黄芩苷可减轻空气栓塞所致急性肺损伤，在这种临床情况下可能被视为一种有用的辅助药物治疗。
