Pan Hung-Chuan, Cheng Fu-Chou, Chen Chun-Jung, Lai Shu-Zhen, Liu Mu-Jung, Chang Ming-Hong, Wang Yeou-Chih, Yang Dar-Yu, Ho Shu-Peng
Department of Neurosurgery, Taichung Veterans General Hospital, Taichung, Taiwan.
Neurol Res. 2009 Jun;31(5):441-52. doi: 10.1179/174313209X403878. Epub 2009 Mar 23.
Clearance of fibrin and associated inflammatory cytokines by tissue-type plasminogen activator (t-PA) is related to improved regeneration in neurological disorder. The biological activity of fermented soybean (natto) is very similar to that of t-PA. We investigated the effect of the dietary supplement of natto on peripheral nerve regeneration. The peripheral nerve injury was produced by crushing the left sciatic nerve with a vessel clamp in Sprague-Dawley rats. The injured animals were fed orally either with saline or natto (16 mg/day) for seven consecutive days after injury. Increased functional outcome such as sciatic nerve functional index, angle of ankle, compound muscle action potential and conduction latency were observed in natto-treated group. Histological examination demonstrated that natto treatment improved injury-induced vacuole formation, S-100 and vessel immunoreactivities and axon loss. Oral intake of natto prolonged prothrombin time and reduced fibrinogen but did not change activated partial thromboplastin time and bleeding time. Furthermore, natto decreased injury-induced fibrin deposition, indicating a tolerant fibrinolytic activity. The treatment of natto significantly improved injury-induced disruption of blood-nerve barrier and loss of matrix component such as laminin and fibronectin. Sciatic nerve crush injury induced elevation of tumor necrosis factor alpha (TNF-alpha) production and caused apoptosis. The increased production of TNF-alpha and apoptosis were attenuated by natto treatment. These findings indicate that oral intake of natto has the potential to augment regeneration in peripheral nerve injury, possibly mediated by the clearance of fibrin and decreased production of TNF-alpha.
组织型纤溶酶原激活剂（t-PA）清除纤维蛋白及相关炎性细胞因子与改善神经疾病的再生有关。发酵大豆（纳豆）的生物活性与t-PA非常相似。我们研究了补充纳豆饮食对周围神经再生的影响。通过用血管夹夹伤Sprague-Dawley大鼠的左侧坐骨神经来造成周围神经损伤。受伤动物在受伤后连续7天口服生理盐水或纳豆（16毫克/天）。在纳豆治疗组中观察到功能结果有所改善，如坐骨神经功能指数、踝关节角度、复合肌肉动作电位和传导潜伏期。组织学检查表明，纳豆治疗改善了损伤诱导的空泡形成、S-100和血管免疫反应以及轴突损失。口服纳豆可延长凝血酶原时间并降低纤维蛋白原，但不改变活化部分凝血活酶时间和出血时间。此外，纳豆减少了损伤诱导的纤维蛋白沉积，表明其具有适度的纤溶活性。纳豆治疗显著改善了损伤诱导的血神经屏障破坏以及层粘连蛋白和纤连蛋白等基质成分的损失。坐骨神经挤压伤导致肿瘤坏死因子α（TNF-α）产生升高并引起细胞凋亡。纳豆治疗可减轻TNF-α产生的增加和细胞凋亡。这些发现表明，口服纳豆有可能增强周围神经损伤的再生，可能是通过清除纤维蛋白和减少TNF-α的产生来介导的。
