C57BL小鼠中X射线诱导白血病的免疫预防
Immunoprevention of x-ray-induced leukemias in the C57BL mouse.
作者信息
Peters R L, Sass B, Stephenson J R, Al-Ghazzouli I K, Hino S, Donahoe R M, Kende M, Aaronson S A, KElloff G J
出版信息
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1697-701. doi: 10.1073/pnas.74.4.1697.
An attempt to prevent irradiation-induced thymic lymphomas in C57BL mice was made by inducing active immunity to endogenous type-C virus with inactivated Rauscher murine leukemia virus (MuLV) or inactivated Gross MuLV or by transferring passive immunity to endogenous type-C virus with goat anti-Gross MuLV IgG. Control groups received the following immunogen or treatment: inactivated simian sarcoma virus, complete Freund's adjuvant, normal goat IgG, and diluent, in both irradiated and nonirradiated C57BL mice Active immunity to the 70,000 molecular weight glycoprotein AKR-gp70 by immunization with Rauscher MuLV and passive immunity to AKR-gp70 by passive transfer of goat anti-Gross-MuLV IgG was measurable throughout some of the latent period of tumor development; in these two groups a significant reduction in tumor incidence was observed, as compared to the other experimental and control groups. Thus, the present findings support the concept of a type-C virus etiology of irradiation-induced leukemias and demonstrate the applicability of immunologic techniques directed against the endogenous type-C virus in the prevention of this disease.
通过用灭活的劳氏鼠白血病病毒（MuLV）或灭活的格罗斯MuLV诱导对内源性C型病毒的主动免疫，或用山羊抗格罗斯MuLV IgG转移对内源性C型病毒的被动免疫，尝试预防C57BL小鼠辐射诱导的胸腺淋巴瘤。对照组接受以下免疫原或处理：灭活的猴肉瘤病毒、完全弗氏佐剂、正常山羊IgG和稀释剂，应用于经辐射和未经辐射的C57BL小鼠。在肿瘤发生的部分潜伏期内，通过用劳氏MuLV免疫可检测到对70000分子量糖蛋白AKR - gp70的主动免疫，通过被动转移山羊抗格罗斯 - MuLV IgG可检测到对AKR - gp70的被动免疫；与其他实验组和对照组相比，这两组的肿瘤发生率显著降低。因此，目前的研究结果支持辐射诱导白血病的C型病毒病因学概念，并证明针对内源性C型病毒的免疫技术在预防该疾病中的适用性。
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