人免疫球蛋白G抗血清胰腺弹性蛋白酶1自身抗体的存在及其对弹性蛋白酶1放射免疫测定的影响。

Presence of human immunoglobulin G anti serum pancreatic elastase 1 autoantibodies and their influence on elastase 1 radioimmunoassay.

作者信息

Asada H, Shibata K, Uchida K, Yamauchi A, Kono M, Takeyama Y, Ohyanagi H, Saitoh Y

机构信息

Shionogi Diagnostics Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.

出版信息

Biochim Biophys Acta. 1991 Oct 11;1080(1):34-9. doi: 10.1016/0167-4838(91)90108-c.

DOI:10.1016/0167-4838(91)90108-c

PMID:1932080

Abstract

Human immunoglobulin G (IgG) anti human pancreatic elastase 1 autoantibodies were detected in sera of patients with pancreatic disorders. The characteristics of these anti elastase 1 autoantibodies and their influence on radioimmunoassay (RIA) for elastase 1 were investigated. They were placed in the IgG class by the double antibody method, and most were assumed to be of a monoclonal type from their elution profiles in gel filtration analysis. The presence of autoantibodies in serum caused an increase in apparent elastase 1 values and a decrease in the recovery of elastase 1 exogenously added to the serum. These results suggest that elastase 1 immunoassay data for autoantibody positive sera can cause misjudgement of clinical stages of patients.

摘要

在胰腺疾病患者的血清中检测到了人免疫球蛋白G（IgG）抗人胰腺弹性蛋白酶1自身抗体。对这些抗弹性蛋白酶1自身抗体的特性及其对弹性蛋白酶1放射免疫测定（RIA）的影响进行了研究。通过双抗体法将它们归类为IgG类，并且根据凝胶过滤分析中的洗脱图谱，大多数被认为是单克隆类型。血清中自身抗体的存在导致表观弹性蛋白酶1值升高，而向血清中外源添加的弹性蛋白酶1回收率降低。这些结果表明，自身抗体阳性血清的弹性蛋白酶1免疫测定数据可能会导致对患者临床分期的错误判断。