Tervaert J W, Mulder L, Stegeman C, Elema J, Huitema M, The H, Kallenberg C
Department of Clinical Immunology, University Hospital, Groningen, The Netherlands.
Ann Rheum Dis. 1993 Feb;52(2):115-20. doi: 10.1136/ard.52.2.115.
Antineutrophil cytoplasmic antibodies (ANCAs) constitute a new class of autoantibodies that seem to recognise myeloid lysosomal enzymes. The occurrence of ANCAs with specificity for human leucocyte elastase (HLE) was assessed in serum samples that were routinely submitted for ANCA determination. During a study period of more than six years anti-HLE was found in only six out of 1102 serum samples that produced a perinuclear or an atypical cytoplasmic staining pattern on ethanol fixed granulocytes. These six serum samples were from four patients with a clinical diagnosis of Wegener's granulomatosis but without a definite histological diagnosis, one patient with systemic vasculitis, and one patient with Cogan's syndrome. To further evaluate the prevalence of anti-HLE we tested 315 serum samples from patients with different forms of vasculitis and related disorders. Anti-HLE was detected in two patients only. Thus autoantibodies to HLE are rarely found in serum samples from patients with vasculitic or related disorders.
抗中性粒细胞胞浆抗体（ANCA）构成了一类新的自身抗体，这类抗体似乎能识别髓系溶酶体酶。在常规送检用于ANCA检测的血清样本中，评估了对人白细胞弹性蛋白酶（HLE）具有特异性的ANCA的出现情况。在超过六年的研究期间，在1102份血清样本中，只有6份在乙醇固定的粒细胞上产生核周或非典型胞浆染色模式的样本中发现了抗HLE。这6份血清样本来自4例临床诊断为韦格纳肉芽肿但无明确组织学诊断的患者、1例系统性血管炎患者和1例科根综合征患者。为了进一步评估抗HLE的患病率，我们检测了315份来自不同形式血管炎和相关疾病患者的血清样本。仅在2例患者中检测到抗HLE。因此，在血管炎或相关疾病患者的血清样本中很少发现抗HLE自身抗体。
