Nakazawa I, Ohneda A, Mura K
Tohoku J Exp Med. 1977 Mar;121(3):275-80. doi: 10.1620/tjem.121.275.
Five cases of malignant insulinoma and 2 cases of benign insulinoma were studied lipid-chemically. Tissues were collected by surgical operation or biopsy under peritoneoscopy. The total lipid was extracted from each tissue, and one part of each total lipid was separated into phospholipid, triglyceride and other lipid fractions by a thin-layer chromatography (TLC) on silica gel. The fatty acid composition and fatty acid content of each lipid fraction were measured by a gas-liquid chromatography (GLC). The most remarkable difference between malignant and benign isulinoma tissues was a higher percentage value of eicosatrienoic acid in the phospholipid of malignant insulinoma tissues when compared with that of non-malignant insulinoma tissues; the values mentioned above distributed between 9.82 and 3.32 in 5 malignant cases, but were 2.89 and 2.57 in 2 benign cases. Those changes in the phospholipid fatty acid composition of malignant insulinoma tissues may represent one of the mechanisms of malignant growth in the malignant neoplastic tissue.
对5例恶性胰岛素瘤和2例良性胰岛素瘤进行了脂质化学研究。组织通过手术切除或腹腔镜活检采集。从每个组织中提取总脂质，然后将每个总脂质的一部分通过硅胶薄层层析（TLC）分离为磷脂、甘油三酯和其他脂质组分。通过气液色谱（GLC）测量每个脂质组分的脂肪酸组成和脂肪酸含量。恶性和良性胰岛素瘤组织之间最显著的差异是，与非恶性胰岛素瘤组织相比，恶性胰岛素瘤组织磷脂中二十碳三烯酸的百分比值更高；上述值在5例恶性病例中分布于9.82至3.32之间，但在2例良性病例中分别为2.89和2.57。恶性胰岛素瘤组织磷脂脂肪酸组成的这些变化可能代表了恶性肿瘤组织恶性生长的机制之一。
