Nakazawa I, Ohtsuki M, Goto Y
Tohoku J Exp Med. 1978 Sep;126(1):95-101. doi: 10.1620/tjem.126.95.
In order to clarify the biochemical features of metastatic tissues into the liver of human cancerous cells, 12 of primary cancerous tissues and 3 of metastatic tissues of the large intestinal cancer, 6 of primary cancerous tissues and 2 of metastatic tissues of the gastric cancer, and 3 of primary cancerous tissues and 3 of metastatic tissues of malignant insulinoma were studied lipid-chemically. Cancerous tissues and metastatic tissues into the liver were collected by biopsy or surgical operation. From each tissue, the total lipid was extracted and one part of the total lipid was separated into phospholipid and triglyceride by TLC. Then, the fatty acid composition and the fatty acid content of each lipid fraction were measured by GLC. The most remarkable findings were recognized in the phospholipid fatty acid composition of the tissues. Namely, the percentage values of C14:0 and C16:1 were larger and that of C20:4 was smaller in metastatic tissues than those of gastric primary lesions. As for the large intestinal cancer, the percentage value of C18:1 was smaller and that of C18:2 larger in metastatic tissues than those of primary lesions. In the malignant insulinoma, the percentage value of C18:0 was larger in metastatic tissues than that of the primary lesions.
为阐明人癌细胞肝转移组织的生化特征，对12例大肠癌原发组织和3例转移组织、6例胃癌原发组织和2例转移组织、3例恶性胰岛素瘤原发组织和3例转移组织进行了脂质化学研究。癌组织和肝转移组织通过活检或手术获取。从每个组织中提取总脂质，总脂质的一部分通过薄层层析法分离为磷脂和甘油三酯。然后，通过气相色谱法测定各脂质组分的脂肪酸组成和脂肪酸含量。在组织的磷脂脂肪酸组成中发现了最显著的结果。即，转移组织中C14:0和C16:1的百分比值较大，而C20:4的百分比值比胃癌原发灶小。对于大肠癌，转移组织中C18:1的百分比值比原发灶小，而C18:2的百分比值比原发灶大。在恶性胰岛素瘤中，转移组织中C18:0的百分比值比原发灶大。
