Nakazawa I, Yamagata S, Watanabe H
Tohoku J Exp Med. 1976;118 Suppl:117-25. doi: 10.1620/tjem.118.suppl_117.
17 cases of cancer and 18 cases of adenomatous polyp in the large intestine were subjected to lipid-chemical studies of their tissues in order to clarify the biochemical differences between malignant neoplastic growth and benign growth. Cancerous and adenomatous polypous tissues were collected by biopsy or surgical operation together with the respective control tissues. One part of the total lipid extracted from each tissue was separated into triglyceride and phospholipid fraction by a thin-layer chromatography (TLC). The fatty acid composition and content of each lipid fraction were measured by a gas-liquid chromatography (GLC). When the fatty acid composition of a phospholipid was expressed in terms of the deviation rate, that of C14:0 was remarkably increased and that of C20:4 was remarkably decreased in the cancerous cases as compared with the respective values of the cases of adenomatous polyp. More definite differences were recognized between the cancerous cases and the cases of adenomatous polyp, when the ratio of the deviation rate of C14:0 to that of C20:4 was calculated in each case. Namely, the ratios distributed between 2.064 and 4.125 in 10 cancerous cases, and between 0.393 and 1.856 in 16 cases of adenomatous polyp, indicating the biochemical differences between cancerous tissues and adenomatous polypous tissues.
为了阐明恶性肿瘤生长与良性生长之间的生化差异，对17例大肠癌和18例大肠腺瘤性息肉的组织进行了脂质化学研究。通过活检或手术采集癌组织和腺瘤性息肉组织以及各自的对照组织。从每个组织中提取的总脂质的一部分通过薄层色谱法（TLC）分离为甘油三酯和磷脂部分。通过气液色谱法（GLC）测量每个脂质部分的脂肪酸组成和含量。当以偏差率表示磷脂的脂肪酸组成时，与腺瘤性息肉病例的相应值相比，癌病例中C14:0的偏差率显著增加，C20:4的偏差率显著降低。当计算每种情况下C14:0的偏差率与C20:4的偏差率之比时，癌病例与腺瘤性息肉病例之间的差异更为明显。即，10例癌病例的比值分布在2.064至4.125之间，16例腺瘤性息肉病例的比值分布在0.393至1.856之间，表明癌组织与腺瘤性息肉组织之间存在生化差异。
