[颌骨区域中央巨细胞病变的DNA细胞光度测定研究]

[DNA cytophotometric studies of central giant-cell lesions of the jaw region].

作者信息

Eckardt A, Pogrel M A, Kaban L B, Chew K, Mayall B H

机构信息

Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Medizinische Hochschule, Hannover, Bundesrepublik Deutschland.

出版信息

Dtsch Zahn Mund Kieferheilkd Zentralbl. 1991;79(6):473-81.

PMID:1932437

Abstract

Giant cell nuclear DNA, in 30 giant cell lesions of the jaws, was quantitated by computer-assisted image analysis. DNA content was then used to predict clinical behavior and outcome. Four.nuclei in each of 25 giant cells (total = 100 nuclei) were randomly selected and DNA content was quantitated by the LEITZ Texture-Analysis-System-Plus. DNA in nuclei of normal appearing stromal fibroblasts (n = 20) was similarly measured. DNA index was calculated as the mean nuclear DNA content of giant cells divided by mean DNA content of control fibroblasts. The mean DNA index of aggressive lesions (1.09, S. D. = 0.12) was not significantly different from that of non-aggressive lesions (1.18, S. D. = 0.15) (p = 0.093). The results indicate that nuclear DNA content of giant cells is not useful as a predictor of clinical behavior of giant-cell lesions of the jaws.

摘要

采用计算机辅助图像分析技术，对30例颌骨巨细胞病变中的巨细胞核DNA进行定量分析。然后将DNA含量用于预测临床行为和预后。从25个巨细胞中随机选取4个细胞核（共100个细胞核），采用LEITZ纹理分析系统升级版对DNA含量进行定量分析。对外观正常的基质成纤维细胞（n = 20）的细胞核DNA进行同样的测量。DNA指数计算为巨细胞平均核DNA含量除以对照成纤维细胞的平均DNA含量。侵袭性病变的平均DNA指数为1.09（标准差= 0.12），与非侵袭性病变（1.18，标准差= 0.15）无显著差异（p = 0.093）。结果表明，巨细胞核DNA含量不能作为颌骨巨细胞病变临床行为的预测指标。