通过流式细胞术表征的耐甲氧西林金黄色葡萄球菌菌株（MRSA）的异质性。
Heterogeneity of methicillin-resistant Staphylococcus aureus strains (MRSA) characterized by flow cytometry.
作者信息
Jarzembowski Tomasz, Wiśniewska Katarzyna, Jóźwik Agnieszka, Witkowski Jacek
机构信息
Department of Medical Microbiology, Medical University of Gdańsk, Str. do Studzienki 38, Gdańsk, Poland.
出版信息
Curr Microbiol. 2009 Jul;59(1):78-80. doi: 10.1007/s00284-009-9395-x. Epub 2009 Mar 28.
We used fluorescent penicillin Bocillin FL for characterization of control methicillin-resistant Staphylococcus aureus (MRSA) strains belonging to one of four heterogenic classes and comparing them with clinical MRSA isolates. Significant differences in percentage of fluorescent cells and reduction of Bocillin FL binding after incubation with methicillin between control strains from classes I and IV were observed, whereas the strains from classes II and III were differed after incubation with methicillin. According to this criteria, 55.8% of the clinical isolates population were similar to the strain of class IV or homogenic resistant, 11.8% was found as I, and 32.3% were categorized as class II or III. However, continuous diversity of measured features was also discussed.
我们使用荧光青霉素Bocillin FL对属于四种异质类之一的对照耐甲氧西林金黄色葡萄球菌（MRSA）菌株进行表征，并将它们与临床MRSA分离株进行比较。观察到I类和IV类对照菌株在与甲氧西林孵育后荧光细胞百分比和Bocillin FL结合减少方面存在显著差异，而II类和III类菌株在与甲氧西林孵育后有所不同。根据这一标准，55.8%的临床分离株群体与IV类菌株或同源抗性菌株相似，11.8%被发现为I类，32.3%被归类为II类或III类。然而，也讨论了测量特征的持续多样性。
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