Goraca Anna, Asłanowicz-Antkowiak Katarzyna
Chair of Experimental and Clinical Physiology, Department of Cardiovascular Physiology, Medical University of Łódź, Mazowiecka 6/8, Łódź, Poland.
Arch Immunol Ther Exp (Warsz). 2009 Mar-Apr;57(2):141-6. doi: 10.1007/s00005-009-0015-z. Epub 2009 Mar 31.
Lipopolysaccharide (LPS) stimulates the synthesis and release of reactive oxygen species that play an important role in the pathogenesis of tissue injuries. In this study the effect of early administration of the antioxidant alpha-lipoic acid (alpha-LA) on brain lipid peroxidation, brain hydrogen peroxide (H(2)O(2)) concentration, and brain total sulfhydryl group (-SH group) content was evaluated in rats with endotoxic shock induced by administration of LPS (Escherichia coli 026:B6, 30 mg/kg i.v.)
Rats were treated intravenously with normal saline or alpha-LA (60 mg/kg) 30 min after LPS injection. After 5 h of observation, the animals were killed and their brains were isolated for the measurements.
Injection of LPS alone resulted in the development of shock and oxidative stress, the latter indicated by a significant increase in brain concentrations of thiobarbituric acid-reacting substances (TBARS) and H(2)O(2) and a decrease in total brain -SH group content. Administration of alpha-LA after the LPS challenge resulted in an increase in total -SH group content and a decrease in TBARS and H(2)O(2) concentration in the brain tissue compared with the LPS group.
The results indicate that alpha-LA treatment effectively protected the brain tissue against endotoxin-induced oxidative stress. Administration of LA could be a useful adjunct to clinical application in the management of septic shock.
脂多糖（LPS）刺激活性氧的合成与释放，活性氧在组织损伤的发病机制中起重要作用。在本研究中，评估了早期给予抗氧化剂α-硫辛酸（α-LA）对注射LPS（大肠杆菌026:B6，30mg/kg静脉注射）诱导的内毒素休克大鼠脑脂质过氧化、脑过氧化氢（H₂O₂）浓度和脑总巯基（-SH基团）含量的影响。
在注射LPS后30分钟，大鼠静脉注射生理盐水或α-LA（60mg/kg）。观察5小时后，处死动物并分离其大脑进行测量。
单独注射LPS导致休克和氧化应激的发生，后者表现为脑硫代巴比妥酸反应物质（TBARS）和H₂O₂浓度显著增加以及脑总-SH基团含量降低。与LPS组相比，在LPS攻击后给予α-LA导致脑组织中总-SH基团含量增加以及TBARS和H₂O₂浓度降低。
结果表明，α-LA治疗可有效保护脑组织免受内毒素诱导的氧化应激。给予硫辛酸可能是脓毒症休克临床治疗中的一种有用辅助手段。
