Department of Pharmaceutical Sciences, St, John's University, College of Pharmacy and Allied Health Professions, 8000 Utopia Parkway, Jamaica, New York 11439, USA.
J Biomed Sci. 2010 Aug 24;17 Suppl 1(Suppl 1):S19. doi: 10.1186/1423-0127-17-S1-S19.
To assess the effect of taurine on lipopolysaccharide (LPS)-induced lung inflammation, oxidative stress and apoptosis, female Golden Syrian hamsters were intratracheally instilled with bacterial LPS (0.02 mg in phosphate buffered saline (PBS) pH 7.4), before or after a 3-day intraperitoneal treatment with a single dose of taurine (50 mg/kg/day in PBS pH 7.4), and bronchoalveolar lavage fluid (BALF) and lung tissue samples were collected at 24 hr after the last treatment. In comparison to BALF samples from animals receiving only PBS pH 7.4, and serving as controls, those of LPS-stimulated animals exhibited a higher count of both total leukocytes and neutrophils and increased expression of tumor necrosis factor receptor 1. In comparison to lungs from control animals, those from LPS-treated animals showed increased cellular apoptosis, lipid peroxidation, decreased glutathione levels, altered activities of antioxidant enzymes (catalase, glutathione peroxidase, superoxide dismutase) and focal inflammation confined to the parenchyma. A treatment with taurine was found to significantly attenuate all these alterations, with the protection being, in all instances, greater when given before rather than after LPS. The present results suggest that taurine is endowed with antiinflammatory and antioxidant properties that are protective in the lung against the deleterious actions of Gram negative bacterial endotoxin.
为了评估牛磺酸对脂多糖（LPS）诱导的肺炎症、氧化应激和细胞凋亡的影响，雌性金黄叙利亚仓鼠经气管内滴注细菌 LPS（0.02mg 于 pH7.4 的磷酸盐缓冲盐水（PBS）中），在 LPS 处理前或后进行为期 3 天的单次腹腔内牛磺酸（50mg/kg/天于 pH7.4 的 PBS 中）治疗，并在最后一次治疗后 24 小时收集支气管肺泡灌洗液（BALF）和肺组织样本。与仅接受 pH7.4 PBS 的 BALF 样本（作为对照）相比，LPS 刺激的动物的总白细胞和中性粒细胞计数均更高，肿瘤坏死因子受体 1 的表达增加。与对照动物的肺相比，LPS 处理的动物的细胞凋亡增加，脂质过氧化增加，谷胱甘肽水平降低，抗氧化酶（过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶）的活性改变，局灶性炎症局限于实质。牛磺酸治疗被发现显著减轻了所有这些改变，并且在 LPS 之前而不是之后给予时，保护作用更大。这些结果表明，牛磺酸具有抗炎和抗氧化特性，可防止革兰氏阴性细菌内毒素对肺的有害作用。
