Amirisetty Ramesh, Patel Ritu Prabha, Das Satrupa, Saraf Jitendra, Jyothy Akka, Munshi Anjana
Chhattisgarh Dental College & Research Institute , Rajnandgaon, Chhattisgarh , India.
Acta Odontol Scand. 2015 Jul;73(5):343-7. doi: 10.3109/00016357.2014.961958. Epub 2014 Sep 25.
Several studies have implicated the role of interleukin-1 in various chronic diseases including periodontitis. The present study was carried out with an aim to evaluate the role of interleukin 1β polymorphisms, namely +3954C/T, -511C/T and -31T/C, in the development of chronic periodontitis.
Twenty-nine chronic periodontitis patients and 31 healthy controls of North Indian origin from Chhattisgarh were recruited for the study. The genotypes for the three variants were determined using the PCR-RFLP technique and the strength of association between genotypes and periodontitis was determined by odds ratio with 95% confidence interval (CI) and chi-square analysis.
Analysis for the +3954 allelic and genotypic frequencies of the polymorphism revealed a significant difference in the CT genotype between periodontitits patients and controls (p = 0.03). A significant difference was also observed in the allelic frequencies between the two groups (p = 0.02). For the -511 site, TT genotype revealed a significant association with the disease (p = 0.01). A significant association was also found following the co-dominant model (p = 0.007). However, the -31 polymorphism revealed no significant difference between patients and controls.
In conclusion, the present study suggests a strong association of the TT genotype of -511 and CT genotype of +3954 variant of interleukin 1β with chronic periodontitis. However, the -31 variant did not show a significant association with the disease.
多项研究表明白细胞介素-1在包括牙周炎在内的多种慢性疾病中发挥作用。本研究旨在评估白细胞介素1β基因多态性，即+3954C/T、-511C/T和-31T/C，在慢性牙周炎发病中的作用。
招募了29名来自恰蒂斯加尔邦的北印度裔慢性牙周炎患者和31名健康对照者进行研究。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术确定这三种变异的基因型，并通过比值比及95%置信区间（CI）和卡方分析确定基因型与牙周炎之间的关联强度。
对该多态性的+3954等位基因和基因型频率分析显示，牙周炎患者和对照者的CT基因型存在显著差异（p = 0.03）。两组之间的等位基因频率也存在显著差异（p = 0.02）。对于-511位点，TT基因型与疾病存在显著关联（p = 0.01）。在共显性模型下也发现了显著关联（p = 0.007）。然而，-31多态性在患者和对照者之间未显示出显著差异。
总之，本研究表明白细胞介素1β的-511 TT基因型和+3954变异的CT基因型与慢性牙周炎存在密切关联。然而，-31变异与该疾病未显示出显著关联。
