Lee Dongwon, Erigala Venkata R, Dasari Madhuri, Yu Junhua, Dickson Robert M, Murthy Niren
The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Int J Nanomedicine. 2008;3(4):471-6.
The overproduction of hydrogen peroxide is implicated in the progress of numerous life-threatening diseases and there is a great need for the development of contrast agents that can detect hydrogen peroxide in vivo. In this communication, we present a new contrast agent for hydrogen peroxide, termed peroxalate micelles, which detect hydrogen peroxide through chemiluminescence, and have the physical/chemical properties needed for in vivo imaging applications. The peroxalate micelles are composed of amphiphilic peroxalate based copolymers and the fluorescent dye rubrene, they have a 'stealth' polyethylene glycol (PEG) corona to evade macrophage phagocytosis, and a diameter of 33 nm to enhance extravasation into permeable tissues. The peroxalate micelles can detect nanomolar concentrations of hydrogen peroxide (>50 nM) and thus have the sensitivity needed to detect physiological concentrations of hydrogen peroxide. We anticipate numerous applications of the peroxalate micelles for in vivo imaging of hydrogen peroxide, given their high sensitivity, small size, and biocompatible PEG corona.
过氧化氢的过量产生与众多危及生命的疾病进展有关，因此迫切需要开发能够在体内检测过氧化氢的造影剂。在本通讯中，我们展示了一种用于过氧化氢的新型造影剂，称为草酸盐胶束，它通过化学发光检测过氧化氢，并具有体内成像应用所需的物理/化学性质。草酸盐胶束由两亲性草酸盐基共聚物和荧光染料红荧烯组成，它们具有“隐形”聚乙二醇（PEG）冠层以逃避巨噬细胞吞噬作用，直径为33nm以增强向可渗透组织的渗出。草酸盐胶束可以检测纳摩尔浓度的过氧化氢（>50 nM），因此具有检测生理浓度过氧化氢所需的灵敏度。鉴于其高灵敏度、小尺寸和生物相容性PEG冠层，我们预计草酸盐胶束在过氧化氢体内成像方面有众多应用。
