Wang Shanfeng, Kempen Diederik H R, Yaszemski Michael J, Lu Lichun
Department of Materials Science and Engineering, The University of Tennessee, Knoxville, 37996, United States.
Biomaterials. 2009 Jul;30(20):3359-70. doi: 10.1016/j.biomaterials.2009.03.015. Epub 2009 Mar 31.
Two poly(ε-caprolactone fumarate)s (PCLFs) with distinct physical properties have been employed to prepare nanocomposites with hydroxyapatite (HA) nanoparticles via photo-crosslinking. The two PCLFs are PCLF530 and PCLF2000, named after their precursor PCL diol molecular weight of 530 and 2000 g.mol, respectively. Crosslinked PCLF530 is amorphous while crosslinked PCLF2000 is semi-crystalline with a melting temperature (T) of ∼40 °C and a crystallinity of 40%. Consequently, the rheological and mechanical properties of crosslinked PCLF2000 are significantly greater than those of crosslinked PCLF530. Structural characterizations and physical properties of both series of crosslinked PCLF/HA nanocomposites with HA compositions of 0%, 5%, 10%, 20%, and 30% have been investigated. By adding HA nanoparticles, crosslinked PCLF530/HA nanocomposites demonstrate enhanced rheological and mechanical properties while the enhancement in compressive modulus is less prominent in crosslinked PCLF2000/HA nanocomposites. cell attachment and proliferation have been performed using rat bone marrow stromal cells (BMSCs) and correlated with the material properties. Cell attachment and proliferation on crosslinked PCLF530/HA nanocomposite disks have been enhanced strongly with increasing the HA composition. However, surface morphology and surface chemistry such as composition, hydrophilicity, and the capability of adsorbing protein cannot be used to interpret the cell responses on different samples. Instead, the role of surface stiffness in regulating cell responses can be supported by the correlation between the change in compressive modulus and BMSC proliferation on these two series of crosslinked PCLFs and PCLF/HA nanocomposites.
两种具有不同物理性质的聚（富马酸ε-己内酯）（PCLFs）已被用于通过光交联制备与羟基磷灰石（HA）纳米颗粒的纳米复合材料。这两种PCLFs分别是PCLF530和PCLF2000，它们以其前体PCL二醇分子量分别为530和2000 g·mol命名。交联的PCLF530是无定形的，而交联的PCLF2000是半结晶的，其熔点（T）约为40°C，结晶度为40%。因此，交联的PCLF2000的流变学和力学性能明显高于交联的PCLF530。研究了HA组成分别为0%、5%、10%、20%和30%的这两个系列交联PCLF/HA纳米复合材料的结构表征和物理性质。通过添加HA纳米颗粒，交联的PCLF530/HA纳米复合材料表现出增强的流变学和力学性能，而交联的PCLF2000/HA纳米复合材料的压缩模量增强则不太显著。使用大鼠骨髓基质细胞（BMSCs）进行了细胞附着和增殖实验，并将其与材料性能相关联。随着HA组成的增加，交联的PCLF530/HA纳米复合盘上的细胞附着和增殖得到了强烈增强。然而，表面形态和表面化学性质，如组成、亲水性和吸附蛋白质的能力，不能用来解释不同样品上的细胞反应。相反，表面硬度在调节细胞反应中的作用可以通过这两个系列交联PCLFs和PCLF/HA纳米复合材料的压缩模量变化与BMSC增殖之间的相关性来支持。
