• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酵母中一种α-淀粉酶超级分泌体的遗传分析；对调控途径的影响。

A genetic analysis of an alpha-amylase super-secretor in yeast; implications for the regulatory pathway.

作者信息

Kotylak Z, el-Gewely M R

机构信息

Department of Biotechnology, University of Tromsø, Norway.

出版信息

Curr Genet. 1991 Aug;20(3):181-4. doi: 10.1007/BF00326229.

DOI:10.1007/BF00326229
PMID:1934124
Abstract

Extracellular glucoamylase activity was increased by a gene, which is present in super-secretor, but absent in low-secretor, strains of the yeast Saccharomyces cerevisiae. Genetic data indicated that this super-secretor gene is linked to the STA3 structural gene for glucoamylase. This gene appears to act specifically since it increased the secretion of glucoamylase but not of other secreted enzymes like acid phosphatase and invertase.

摘要

一种基因可提高细胞外葡糖淀粉酶的活性，该基因存在于酿酒酵母的超分泌型菌株中，但在低分泌型菌株中不存在。遗传数据表明，这种超分泌型基因与葡糖淀粉酶的STA3结构基因相连。该基因似乎具有特异性作用，因为它增加了葡糖淀粉酶的分泌，但没有增加酸性磷酸酶和转化酶等其他分泌酶的分泌。

相似文献

1
A genetic analysis of an alpha-amylase super-secretor in yeast; implications for the regulatory pathway.酵母中一种α-淀粉酶超级分泌体的遗传分析；对调控途径的影响。
Curr Genet. 1991 Aug;20(3):181-4. doi: 10.1007/BF00326229.
2
One-step enzymatic hydrolysis of starch using a recombinant strain of Saccharomyces cerevisiae producing alpha-amylase, glucoamylase and pullulanase.利用产α-淀粉酶、糖化酶和普鲁兰酶的酿酒酵母重组菌株对淀粉进行一步酶促水解。
Appl Microbiol Biotechnol. 1995 Mar;42(6):878-83. doi: 10.1007/BF00191185.
3
Construction of an alpha-amylase/glucoamylase fusion gene and its expression in Saccharomyces cerevisiae.α-淀粉酶/糖化酶融合基因的构建及其在酿酒酵母中的表达。
Biosci Biotechnol Biochem. 1992 Jun;56(6):884-9. doi: 10.1271/bbb.56.884.
4
High-efficiency, one-step starch utilization by transformed Saccharomyces cells which secrete both yeast glucoamylase and mouse alpha-amylase.通过分泌酵母糖化酶和小鼠α淀粉酶的转化酿酒酵母细胞实现高效一步淀粉利用。
Appl Environ Microbiol. 1988 Apr;54(4):966-71. doi: 10.1128/aem.54.4.966-971.1988.
5
Repeated fermentation from raw starch using Saccharomyces cerevisiae displaying both glucoamylase and α-amylase.使用同时展示出糖化酶和α-淀粉酶的酿酒酵母对生淀粉进行重复发酵。
Enzyme Microb Technol. 2012 May 10;50(6-7):343-7. doi: 10.1016/j.enzmictec.2012.03.005. Epub 2012 Mar 22.
6
Co-expression of a Saccharomyces diastaticus glucoamylase-encoding gene and a Bacillus amyloliquefaciens alpha-amylase-encoding gene in Saccharomyces cerevisiae.糖化酵母葡糖淀粉酶编码基因与解淀粉芽孢杆菌α-淀粉酶编码基因在酿酒酵母中的共表达。
Gene. 1991 Apr;100:85-93. doi: 10.1016/0378-1119(91)90353-d.
7
Expression and comparison of codon optimised Aspergillus tubingensis amylase variants in Saccharomyces cerevisiae.在酿酒酵母中表达和比较经过密码子优化的曲霉菌淀粉酶变体。
FEMS Yeast Res. 2017 Jun 1;17(4). doi: 10.1093/femsyr/fox040.
8
Raw starch fermentation to ethanol by an industrial distiller's yeast strain of Saccharomyces cerevisiae expressing glucoamylase and α-amylase genes.利用表达葡糖淀粉酶和α-淀粉酶基因的工业酿酒酵母菌株对生淀粉进行乙醇发酵。
Biotechnol Lett. 2011 Aug;33(8):1643-8. doi: 10.1007/s10529-011-0613-9. Epub 2011 Apr 9.
9
Efficient co-displaying and artificial ratio control of α-amylase and glucoamylase on the yeast cell surface by using combinations of different anchoring domains.通过使用不同的锚定域组合，在酵母细胞表面上有效共展示和人工控制α-淀粉酶和葡萄糖淀粉酶的比例。
Appl Microbiol Biotechnol. 2015 Feb;99(4):1655-63. doi: 10.1007/s00253-014-6250-1. Epub 2014 Nov 30.
10
Consolidated bioprocessing of raw starch with Saccharomyces cerevisiae strains expressing fungal alpha-amylase and glucoamylase combinations.利用表达真菌α-淀粉酶和葡萄糖淀粉酶组合的酿酒酵母菌株对生淀粉进行综合生物加工。
FEMS Yeast Res. 2018 Nov 1;18(7). doi: 10.1093/femsyr/foy085.

本文引用的文献

1
STA10: A gene involved in the control of starch utilization by Saccharomyces.STA10：一个参与控制酿酒酵母淀粉利用的基因。
Curr Genet. 1983 Apr;7(2):109-12. doi: 10.1007/BF00365634.
2
A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts.对酵母分类中常用的氮同化试验的批判性评价。
J Bacteriol. 1946 Sep;52(3):293-301.
3
Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway.酵母分泌途径中翻译后事件所需的23个互补组的鉴定。
Cell. 1980 Aug;21(1):205-15. doi: 10.1016/0092-8674(80)90128-2.
4
Intracellular transport of secretory proteins in the pancreatic exocrine cell. II. Transport to condensing vacuoles and zymogen granules.胰腺外分泌细胞中分泌蛋白的细胞内运输。II. 向浓缩泡和酶原颗粒的运输。
J Cell Biol. 1967 Aug;34(2):597-615. doi: 10.1083/jcb.34.2.597.
5
Genetic mapping of nonsense suppressors in yeast.酵母中无义抑制基因的遗传图谱
Genetics. 1968 Dec;60(4):735-42. doi: 10.1093/genetics/60.4.735.
6
Regulation and characterization of acid and alkaline phosphatase in yeast.酵母中酸性和碱性磷酸酶的调控与特性研究
J Gen Microbiol. 1971 Mar;65(3):291-303. doi: 10.1099/00221287-65-3-291.
7
Heterologous protein secretion from yeast.酵母中异源蛋白的分泌
Science. 1985 Sep 20;229(4719):1219-24. doi: 10.1126/science.3939723.
8
Control of STA1 gene expression by the mating-type locus in yeasts.酵母中交配型基因座对STA1基因表达的调控。
J Bacteriol. 1985 Nov;164(2):769-73. doi: 10.1128/jb.164.2.769-773.1985.
9
Isolation and characterization of mutants which show an oversecretion phenotype in Saccharomyces cerevisiae.酿酒酵母中呈现过分泌表型的突变体的分离与鉴定。
Genetics. 1988 Jul;119(3):499-506. doi: 10.1093/genetics/119.3.499.
10
Regulated overproduction of alpha-amylase by transformation of the amylolytic yeast Schwanniomyces occidentalis.通过转化解脂酵母西方许旺酵母来调控α-淀粉酶的过量生产。
Curr Genet. 1989 May;15(5):319-25. doi: 10.1007/BF00419911.