Vreman Hendrik J, Knauer Yuri, Wong Ronald J, Chan Miu-Lan, Stevenson David K
Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
Pediatr Res. 2009 Jul;66(1):66-9. doi: 10.1203/PDR.0b013e3181a7be77.
Total body, head, and trunk carbon monoxide (CO) excretion rates were measured separately by gas chromatography in 1- to 7-d-old Wistar rat pups exposed to the dark and to mixed blue (one Special Blue-F20T12/BB) and white (two Cool White-F20T12/CW) fluorescent light or blue light emitting diode (LED) sources. During 48-min cycled exposures to the dark and to either light source, total body CO excretion rapidly increased 1.9- and 1.4-fold, respectively, over dark control levels. When CO excretion rates from the head and trunk were measured separately during exposure to either light source, CO excretion from the head did not change significantly; however, a large mean 4.4-fold increase in CO excretion from the trunk was observed. When light intensity delivered by the blue LED source was varied, we found that trunk CO excretion increased with increasing light intensities. In the presence of riboflavin (10 micromol/kg), total body CO excretion increased 2.8- and 2.1-fold during exposure to the mixed fluorescent light and blue LED sources, respectively. We conclude that light-induced elevations in total body CO excretion may be caused by transdermally excreted CO, which is most likely produced through endogenous photosensitizer-mediated photooxidation of dermal biomolecules.
通过气相色谱法分别测量了1至7日龄的Wistar大鼠幼崽在黑暗环境以及暴露于混合蓝光（一个特殊蓝光-F20T12/BB）和白光（两个冷白光-F20T12/CW）荧光光源或蓝光发光二极管（LED）光源下时的全身、头部和躯干一氧化碳（CO）排泄率。在48分钟的黑暗与任一光源交替暴露过程中，全身CO排泄量分别比黑暗对照组水平迅速增加了1.9倍和1.4倍。当在暴露于任一光源期间分别测量头部和躯干的CO排泄率时，头部的CO排泄没有显著变化；然而，观察到躯干的CO排泄平均大幅增加了4.4倍。当改变蓝光LED光源的光强度时，我们发现躯干CO排泄量随光强度增加而增加。在存在核黄素（10微摩尔/千克）的情况下，在暴露于混合荧光光源和蓝光LED光源期间，全身CO排泄量分别增加了2.8倍和2.1倍。我们得出结论，光诱导的全身CO排泄增加可能是由经皮排泄的CO引起的，而CO很可能是通过内源性光敏剂介导的皮肤生物分子光氧化产生的。
