Kim Jong-Hak, Kim Min-Kyeoung, Wang Hongtao, Lee Hee-Nyeong, Jin Chi-Gyu, Kwon Woo-Saeng, Yang Deok-Chun
Department of Oriental Medicinal Material & Processing, College of Life Sciences, Kyung Hee University, Yongin, Korea.
KM Fundamental Research Division, Korea Institute of Oriental Medicine, Daejeon, Korea.
J Ginseng Res. 2016 Oct;40(4):395-399. doi: 10.1016/j.jgr.2015.12.002. Epub 2015 Dec 17.
Korean ginseng () is one of the most important medicinal plants in the Orient. Among nine cultivars of , Chunpoong commands a much greater market value and has been planted widely in Korea. Chunpoong has superior quality "Chunsam" (1 grade ginseng) when made into red ginseng.
A rapid and reliable method for discriminating the Chunpoong cultivar was developed by exploiting a single nucleotide polymorphism (SNP) in the gene of nine Korean ginseng cultivars using specific primers.
An SNP was detected between Chunpoong and other cultivars, and modified allele-specific primers were designed from this SNP site to specifically identify the Chunpoong cultivar and via multiplex polymerase chain reaction (PCR).
These results suggest that great impact to prevent authentication of precise Chunpoong and other cultivars using the gene. We therefore present an effective method for the authentication of the Chunpoong cultivar of and .
高丽参是东方最重要的药用植物之一。在高丽参的九个栽培品种中，春蓬的市场价值更高，已在韩国广泛种植。春蓬制成红参后具有优质的“春三”（一级人参）。
利用特异性引物，通过检测九个高丽参栽培品种基因中的单核苷酸多态性（SNP），开发了一种快速可靠的鉴别春蓬品种的方法。
在春蓬和其他品种之间检测到一个SNP，并从该SNP位点设计了修饰的等位基因特异性引物，通过多重聚合酶链反应（PCR）特异性鉴定春蓬品种和。
这些结果表明，利用基因对准确鉴别春蓬和其他品种有很大影响。因此，我们提出了一种鉴别高丽参和春蓬品种的有效方法。
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