Robinson Stephen J, Parkin Isobel A P
Agriculure and Agri-food, Saskatoon Research Centre, Saskatoon, SK, Canada.
Methods Mol Biol. 2009;513:153-73. doi: 10.1007/978-1-59745-427-8_9.
The explosion of genomics data has led to a significant knowledge gap, with thousands of genes identified having no known function. The following chapter describes the available forward and reverse genetics strategies, which can assist researchers in assigning functions to novel genes. Details of the available resources for a number of model and crop species are provided. In addition, protocols are presented for utilising T-DNA tagged populations to identify genes underlying novel phenotypes and to assist with functional characterisation of target genes.
基因组学数据的爆炸式增长导致了巨大的知识缺口，数以千计已鉴定的基因尚无已知功能。以下章节描述了现有的正向和反向遗传学策略，这些策略可帮助研究人员为新基因赋予功能。文中提供了多种模式生物和作物物种的可用资源详情。此外，还介绍了利用T-DNA标签群体来鉴定新表型背后的基因以及辅助目标基因功能表征的实验方案。
