Dipartimento di Scienza e Tecnologia del Farmaco University of Torino, Torino, Italy.
Planta Med. 2009 Oct;75(12):1331-5. doi: 10.1055/s-0029-1185546. Epub 2009 Apr 3.
The essential oils of Ridolfia segetum (L.) Moris and Oenanthe crocata L. (Apiaceae), collected in Sardinia (Italy), have been assayed for two enzyme-associated activities of the HIV-1 reverse transcriptase (RT): RNA-dependent DNA polymerase (RDDP) activity and ribonuclease H (RNase H) activity. In biochemical assays, the essential oils inhibited HIV-1 RT RDDP activity in a dose-dependent manner, while they were inactive towards RNase H activity. Furthermore, the oils were cytotoxic towards K (562) cell replication. GC-MS analysis of the essential oils obtained by steam distillation of the aerial parts showed that the main components of R. segetum were alpha-phellandrene, alpha-terpinolene, beta-phellandrene, and dillapiol and those of O. crocata were sabinene, TRANS-beta-ocimene, CIS-beta-ocimene, and beta-pinene.
里德利福蒂亚属植物（L.）Moris 和欧芹属植物（L.）（伞形科）的精油，在撒丁岛（意大利）采集，已经对 HIV-1 逆转录酶（RT）的两种酶相关活性进行了检测：RNA 依赖性 DNA 聚合酶（RDDP）活性和核糖核酸酶 H（RNase H）活性。在生化测定中，精油以剂量依赖性方式抑制 HIV-1 RT RDDP 活性，而对 RNase H 活性无活性。此外，这些油对 K（562）细胞复制具有细胞毒性。通过水蒸气蒸馏从地上部分获得的精油的 GC-MS 分析表明，里德利福蒂亚属植物的主要成分是α-水芹烯、α-松油醇、β-水芹烯和大根香叶醇，而欧芹属植物的主要成分是柠檬烯、反式-β-罗勒烯、顺式-β-罗勒烯和β-蒎烯。
