El Karkouri Jamila, Kchibale Amale, Chroho Mounia, Eddamsyry Brahim, Touijer Hanane, El Makhoukhi Fadoua, Handaq Nadia, Eto Bruno, Salamatullah Ahmad Mohammad, Bourhia Mohammed, Zair Touriya
Research Team of Chemistry of Bioactive Molecules and the Environment, Laboratory of Innovative Materials and Biotechnology of Natural Resources, Faculty of Sciences, Moulay Ismaïl University, B.P. 11201 Zitoune, Meknes 50070, Morocco.
Laboratoires TBC, Laboratory of Pharmacology, Pharmacokinetics and Clinical Pharmacy, Faculty of Pharmacy, University of Lille, 3, Rue du Professeur Laguesse, B.P. 83, F-59000 Lille, France.
Life (Basel). 2022 Dec 23;13(1):44. doi: 10.3390/life13010044.
The present work was designed to study the chemical composition, antioxidant, antihyperglycemic effect, and toxicity assessment of (L.) Moris extract. The chemical composition was studied by use of high-performance liquid chromatography (HPLC). Antioxidant power was tested by use of DPPH and FRAP assays. The antihyperglycemic effect was tested by use of a glucose tolerance test, while toxicity assessment was done in vivo by use of Wistar rats for 14 days. Analysis of the extract by HPLC-UV revealed the presence of gallic acid, catechol, vanillic acid, catechin, tannic acid, rosmarinic acid, naringenin, and coumarin acid. The crude hydroethanolic extract possessed high levels of total phenols (15.6 ± 1.76 mg EAG/g), condensed tannins (383.49 mg ECat/g DM), and flavonoid (11.63 mg EQ/g). The findings showed that the studied extract possessed good antioxidant power with IC values equal to 550, 650, 700 µg/mL respectively for the decoction, the ethyl acetate fraction (F), and the ethyl acetate fraction (F). For the antioxidant activity by FRAP, the aqueous fraction (F) and the aqueous extract (F) showed CE values of 0.33 mg/mL and 0.4 mg/mL, respectively. Glucose tolerance test analysis showed that (L.) Moris decoction had a significant postprandial antihyperglycemic effect in normal Wistar rats. The results of the acute toxicity test showed that the decoction was not toxic even at 2 g/Kg. Pancreatic α-amylase activity was significantly inhibited in the presence of (L.) Moris extract (IC = 0.133 ± 0.09 mg/mL). The outcome of the present work showed that (L.) Moris is very rich in phenolic compounds with potent antioxidant and antihyperglycemic effects.
本研究旨在探讨(L.) Moris提取物的化学成分、抗氧化、降血糖作用及毒性评估。采用高效液相色谱法(HPLC)研究其化学成分。通过DPPH和FRAP试验检测抗氧化能力。通过葡萄糖耐量试验检测降血糖作用，同时使用Wistar大鼠进行14天的体内毒性评估。通过HPLC-UV分析提取物，发现其中含有没食子酸、儿茶酚、香草酸、儿茶素、单宁酸、迷迭香酸、柚皮素和香豆素酸。粗制水乙醇提取物中总酚含量较高(15.6±1.76 mg EAG/g)、缩合单宁含量较高(383.49 mg ECat/g DM)和黄酮含量较高(11.63 mg EQ/g)。研究结果表明，所研究的提取物具有良好的抗氧化能力，其煎剂、乙酸乙酯部分(F)和乙酸乙酯部分(F)的IC值分别为550、650、700 μg/mL。对于FRAP抗氧化活性，水相部分(F)和水提取物(F)的CE值分别为0.33 mg/mL和0.4 mg/mL。葡萄糖耐量试验分析表明，(L.) Moris煎剂对正常Wistar大鼠具有显著的餐后降血糖作用。急性毒性试验结果表明，即使剂量为2 g/Kg，该煎剂也无毒。在(L.) Moris提取物存在的情况下，胰腺α-淀粉酶活性受到显著抑制(IC = 0.133±0.09 mg/mL)。本研究结果表明，(L.) Moris富含具有强大抗氧化和降血糖作用的酚类化合物。
