Yuzbasioglu Mehmet Fatih, Ezberci Fikret, Senoglu Nimet, Ciragil Pinar, Tolun Fatma Inanc, Oksuz Hafize, Cetinkaya Ali, Atli Yalcin, Kale Ilhami Taner
Department of General Surgery, Faculty of Medicine, Kahramanmaras Sutcuimam University, Kahramanmaras, Turkey.
Bratisl Lek Listy. 2008;109(12):537-43.
The accelerative effect of EMLA (eutectic mixture of lidocaine 2.5% and prilocaine 2.5%) in the wound healing process is known. We hypothesised that post-operative peritoneal adhesions may be reduced with intra-peritoneal EMLA administration in a model of bacterial peritonitis.
Bacterial peritonitis was induced in 24 rats by cecal ligation and puncture. The rats were randomly assigned to one of four groups. Group 1 (n=6)) received EMLA intraperitoneally, group 2 (n=6) received 2% lidocaine hcl solution intraperitoneally, the third group received one dose (100 mg/kg) of ceftriaxone sodium (Rocephin, Roche, 1 g) intraperitoneally one day after cecal ligation and puncture procedure, and in control group (group 4, n=6), no fluid or medicine was introduced into the abdomens of the rats. All animals were killed 14 days later in order to assess the adhesion score. Tissue antioxidant levels were measured in 1 g tissue samples taken from the abdominal wall.
The adhesion score was significantly lower in the EMLA group than in the lidocaine and control groups. The catalase levels were higher in the lidocaine and control groups than in EMLA group.
Intraperitoneal EMLA inhibited the formation of postoperative intra-abdominal adhesions without compromising the wound healing in this bacterial peritonitis rat model. EMLA also decreased the oxidative stress during peritonitis (Tab. 1, Fig. 7, Ref. 27). Full Text (Free, PDF) www.bmj.sk.
已知EMLA（2.5%利多卡因与2.5%丙胺卡因的共熔混合物）对伤口愈合过程有促进作用。我们推测在细菌性腹膜炎模型中，腹腔内给予EMLA可减少术后腹膜粘连。
通过盲肠结扎和穿刺在24只大鼠中诱发细菌性腹膜炎。将大鼠随机分为四组。第1组（n = 6）腹腔内给予EMLA，第2组（n = 6）腹腔内给予2%盐酸利多卡因溶液，第三组在盲肠结扎和穿刺术后一天腹腔内给予一剂（100 mg/kg）头孢曲松钠（罗氏芬，罗氏公司，1 g），对照组（第4组，n = 6）大鼠腹腔内不注入液体或药物。14天后处死所有动物以评估粘连评分。从腹壁采集1 g组织样本测量组织抗氧化水平。
EMLA组的粘连评分显著低于利多卡因组和对照组。利多卡因组和对照组的过氧化氢酶水平高于EMLA组。
在该细菌性腹膜炎大鼠模型中，腹腔内给予EMLA可抑制术后腹腔内粘连的形成，且不影响伤口愈合。EMLA还可降低腹膜炎期间的氧化应激（表1，图7，参考文献27）。全文（免费，PDF）www.bmj.sk 。
