Zucolotto Silvana Maria, Goulart Stella, Montanher Ana B, Reginatto Flávio Henrique, Schenkel Eloir Paulo, Fröde Tânia Silvia
Department of Pharmaceutical Science, Center of Health Sciences, Federal University of Santa Catarina, Campus, Universitário, Trindade, Florianópolis, SC, Brazil.
Planta Med. 2009 Sep;75(11):1221-6. doi: 10.1055/s-0029-1185536. Epub 2009 Apr 7.
Passiflora edulis, commonly known as "maracujá", is widely cultivated in Brazil for the industrial production of juice. The species of Passiflora are popularly used as a sedative or tranquillizer, and also against intermittent fever and skin inflammation. In this study we evaluated the anti-inflammatory activity of four sub-fractions and three isolated compounds from the butanolic fraction of P. edulis var. flavicarpa leaves, using the mouse model of pleurisy induced by carrageenan. The butanolic fraction obtained from an aqueous extract of P. edulis (50 and 100 mg/kg, I. P.) showed anti-inflammatory activity by inhibiting leukocytes and neutrophils (p < 0.01). Sub-fraction C showed itself to be more effective than the other sub-fractions (p < 0.01). Isoorientin ( 1), vicenin-2 ( 2) and spinosin ( 3) were isolated from the active sub-fraction C derived from the butanolic fraction. The sub-fraction C (50 mg/kg, I. P.), as well as its major isolated compounds (25 mg/kg, I. P.), inhibited leukocytes and neutrophils (p < 0.05). Additionally, the butanolic fraction and isoorientin also inhibited myeloperoxidase activity (p < 0.05). The present study showed that the C-glucosylflavones isolated from P. edulis leaves can be responsible for the anti-inflammatory effect of P. edulis on the mouse model of pleurisy.
西番莲，通常被称为“maracujá”，在巴西广泛种植用于果汁的工业生产。西番莲属植物通常被用作镇静剂或镇定剂，也用于治疗间歇热和皮肤炎症。在本研究中，我们使用角叉菜胶诱导的小鼠胸膜炎模型，评估了西番莲黄果变种叶片丁醇部位的四个亚组分和三个分离化合物的抗炎活性。从西番莲水提取物中获得的丁醇部位（50和100mg/kg，腹腔注射）通过抑制白细胞和中性粒细胞显示出抗炎活性（p<0.01）。亚组分C比其他亚组分更有效（p<0.01）。异荭草素（1）、异荭草苷-2（2）和斯皮诺素（3）从丁醇部位的活性亚组分C中分离得到。亚组分C（50mg/kg，腹腔注射）及其主要分离化合物（25mg/kg，腹腔注射）抑制白细胞和中性粒细胞（p<0.05）。此外，丁醇部位和异荭草素也抑制髓过氧化物酶活性（p<0.05）。本研究表明，从西番莲叶片中分离得到的C-葡萄糖基黄酮可能是西番莲对小鼠胸膜炎模型抗炎作用的原因。
