Huang D P, Ho H C, Ng M H, Lui M
Br J Cancer. 1977 May;35(5):630-4. doi: 10.1038/bjc.1977.98.
Explants of fresh biopsy specimens from non-neoplastic nasopharyngeal (NP) mucosa, nasopharyngeal carcinoma (NPC), other tumours (OT) of the head and neck and freshly removed tonsils were treated with an Epstein-Barr virus (EBV) preparation from B95-8 cells and cultured. The mainly epitheloid outgrowths from these infected explants were then compared with those from their respective uninfected controls at 14 days. Growth stimulation occurred with a significantly higher frequency, and the degree of stimulation was generally higher with the infected NP explants than those of the similarly infected explants of other origins. Furthermore, after treatment with the virus preparation, several of the outgrowths from the NP explants showed growth characteristics and cellular morphology typical of those of transformed cells. Light microscopy has shown the changed NP cells to have epithelial characteristics. This is now being verified by electron microscopy, which has so far shown the presence of keratin fibrils and desmosomes in one specimen examined. They are also being examined for the presence of EBV-DNA and EBNA, and other features of transformation, including malignant tendency, by passage through athymic nude mice.
取自非肿瘤性鼻咽（NP）黏膜、鼻咽癌（NPC）、头颈部其他肿瘤（OT）的新鲜活检标本外植体以及新鲜切除的扁桃体，用来自B95 - 8细胞的爱泼斯坦 - 巴尔病毒（EBV）制剂处理后进行培养。然后在14天时，将这些受感染外植体长出的主要上皮样细胞与各自未感染的对照进行比较。生长刺激出现的频率显著更高，并且受感染的NP外植体的刺激程度通常高于其他来源的类似受感染外植体。此外，用病毒制剂处理后，NP外植体长出的一些细胞表现出转化细胞典型的生长特征和细胞形态。光学显微镜显示变化后的NP细胞具有上皮特征。目前正在通过电子显微镜进行验证，到目前为止，在一个检查的标本中已发现角蛋白纤维和桥粒的存在。还通过将其接种无胸腺裸鼠，检查它们是否存在EBV - DNA和EBNA以及其他转化特征，包括恶性倾向。
