血浆或红细胞磷脂可用于评估孕期女性的二十二碳六烯酸状态。

Plasma or red blood cell phospholipids can be used to assess docosahexaenoic acid status in women during pregnancy.

作者信息

Courville Amber B, Keplinger Melissa R, Judge Michelle P, Lammi-Keefe Carol J

机构信息

University of Connecticut Department of Nutritional Sciences, Storrs, CT, USA.

出版信息

Nutr Res. 2009 Mar;29(3):151-5. doi: 10.1016/j.nutres.2009.02.005.

DOI:10.1016/j.nutres.2009.02.005

PMID:19358928

Abstract

The suitability of using plasma phopholipids (PLs) to assess docosahexaenoic acid (DHA) status during pregnancy is well accepted. Recent discussions have centered around whether red blood cells (RBCs) can be used to indicate DHA status. We tested the hypothesis that in pregnant women participating in an intervention study when fed a functional food containing DHA, maternal plasma PL DHA would be positively associated with maternal RBC PL and umbilical cord blood RBC PL DHA. Maternal and umbilical cord blood samples were obtained at delivery from women whose mean dietary intake was 187 mg/d (including the amount consumed from the DHA-functional food). Maternal plasma and RBCs and cord blood RBC lipids were extracted and PLs separated by thin-layer chromatography. Phopholipid lipids were methylated, and fatty acids were identified using gas chromatography. Fifty-nine maternal samples and 30 cord blood samples were analyzed. There were moderate to strong correlations between DHA in all compartments (maternal plasma vs maternal RBC PL DHA weight percent [wt%], r = 0.633, P < or = .001; maternal plasma vs cord blood RBC PL DHA wt%, r = 0.458, P < or = .01; maternal RBCs vs cord blood RBC PL DHA wt%, r = 0.376, P < or = .01). These results support the practice of using either plasma PLs or RBC PLs to assess maternal and infant DHA status.

摘要

在孕期使用血浆磷脂（PLs）来评估二十二碳六烯酸（DHA）状态的适用性已得到广泛认可。最近的讨论集中在红细胞（RBCs）是否可用于指示DHA状态。我们检验了这样一个假设：在参与一项干预研究的孕妇中，当她们食用含DHA的功能性食品时，母体血浆PL DHA会与母体RBC PL以及脐带血RBC PL DHA呈正相关。在分娩时从平均饮食摄入量为187毫克/天（包括从DHA功能性食品中摄入的量）的女性身上采集母体和脐带血样本。提取母体血浆和RBCs以及脐带血RBC脂质，并通过薄层色谱法分离PLs。将磷脂脂质甲基化，并用气相色谱法鉴定脂肪酸。分析了59份母体样本和30份脐带血样本。所有组分中的DHA之间存在中度至强相关性（母体血浆与母体RBC PL DHA重量百分比[wt%]，r = 0.633，P≤0.001；母体血浆与脐带血RBC PL DHA wt%，r = 0.458，P≤0.01；母体RBCs与脐带血RBC PL DHA wt%，r = 0.376，P≤0.01）。这些结果支持使用血浆PLs或RBC PLs来评估母体和婴儿DHA状态的做法。