Osaka Tetsuya, Nakanishi Takuya, Shanmugam Sangaraju, Takahama Shintaro, Zhang Hong
School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
Colloids Surf B Biointerfaces. 2009 Jul 1;71(2):325-30. doi: 10.1016/j.colsurfb.2009.03.004. Epub 2009 Mar 24.
Internalization of magnetite nanoparticles with diameter of approximately 40 nm into normal and cancer cells was examined by microscopic observation and flow cytometry. Magnetite nanoparticles were synthesized by hydrolysis in an aqueous solution containing ferrous chloride with organic amines as a base. It was demonstrated that the difference in surface charge of magnetite nanoparticles brought about the difference in uptake efficiency. The nanoparticles with positive charge showed higher internalization into human breast cancer cells than the nanoparticles with negative charge, while the degree of internalization of the positively- and negatively-charged nanoparticles into human umbilical vein endothelial cells (HUVEC) was almost the same.
通过显微镜观察和流式细胞术检测了直径约40 nm的磁铁矿纳米颗粒进入正常细胞和癌细胞的内化情况。磁铁矿纳米颗粒是在含有氯化亚铁和有机胺作为碱的水溶液中通过水解合成的。结果表明，磁铁矿纳米颗粒表面电荷的差异导致了摄取效率的差异。带正电荷的纳米颗粒比带负电荷的纳米颗粒在人乳腺癌细胞中的内化程度更高，而带正电荷和负电荷的纳米颗粒在人脐静脉内皮细胞（HUVEC）中的内化程度几乎相同。
