聚酮合酶的结构酶学
Structural enzymology of polyketide synthases.
作者信息
Tsai Shiou-Chuan Sheryl, Ames Brian Douglas
机构信息
Department of Molecular Biology, University of California, Irvine, California, USA.
出版信息
Methods Enzymol. 2009;459:17-47. doi: 10.1016/S0076-6879(09)04602-3.
Abstract
This chapter describes structural and associated enzymological studies of polyketide synthases, including isolated single domains and multidomain fragments. The sequence-structure-function relationship of polyketide biosynthesis, compared with homologous fatty acid synthesis, is discussed in detail. Structural enzymology sheds light on sequence and structural motifs that are important for the precise timing, substrate recognition, enzyme catalysis, and protein-protein interactions leading to the extraordinary structural diversity of naturally occurring polyketides.
摘要
本章描述了聚酮合酶的结构及相关酶学研究，包括分离出的单个结构域和多结构域片段。详细讨论了聚酮生物合成与同源脂肪酸合成相比的序列-结构-功能关系。结构酶学揭示了对于精确计时、底物识别、酶催化以及导致天然聚酮化合物具有非凡结构多样性的蛋白质-蛋白质相互作用而言重要的序列和结构基序。
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