Li Yan-Chao, Bai Wan-Zhu, Sakai Kazuhisa, Hashikawa Tsutomu
Neural Architecture, Advanced Technology Development Group, RIKEN Brain Science Institute, Hirosawa 2-1, Wako, Saitama 351-0198, Japan.
J Histochem Cytochem. 2009 Aug;57(8):741-51. doi: 10.1369/jhc.2009.953646. Epub 2009 Apr 13.
The organization of F-actin in the ventricular system has been reported to display pronounced regional differences with respect to shape, size, and development. However, the real roles played by F-actin in these cells cannot be understood unless the precise localization of F-actin is defined. In the present study, we used double-fluorescence labeling to further examine the localization of F-actin in the ependymocytes and its spatial relation to the other two cytoskeletal components, microtubules and intermediate filaments. Then we converted fluorescence signals for F-actin to peroxidase/DAB reaction products by use of a phalloidin-based FITC-anti-FITC system. This detection technique provided an overview of the distribution of F-actin in the ependymocytes at the ultrastructural level, and has been proven to be helpful in correlating light and electron microscopic investigations.
据报道，心室系统中F-肌动蛋白的组织在形状、大小和发育方面存在明显的区域差异。然而，除非确定F-肌动蛋白的精确定位，否则无法理解其在这些细胞中所起的实际作用。在本研究中，我们使用双重荧光标记进一步研究F-肌动蛋白在室管膜细胞中的定位及其与其他两种细胞骨架成分微管和中间丝的空间关系。然后，我们通过基于鬼笔环肽的FITC-抗FITC系统将F-肌动蛋白的荧光信号转化为过氧化物酶/DAB反应产物。这种检测技术在超微结构水平上提供了F-肌动蛋白在室管膜细胞中分布的概况，并已被证明有助于关联光学和电子显微镜研究。
