Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India.
Phytother Res. 2009 Nov;23(11):1565-74. doi: 10.1002/ptr.2822.
The present study was designed to investigate the ameliorative potential of Punica granatum in dextran sulfate sodium (DSS) induced ulcerative colitis. DSS (2%) was administered orally in drinking water for 7 days to induce ulcerative colitis. The extent and severity of ulceration was analysed macroscopically, histopathologically and using a disease activity index. Myeloperoxidase (MPO), a specific marker of inflammation; histamine, a marker of mast cell degranulation; superoxide anion generation and, lipid peroxides were analysed. Administration of DSS resulted in a significant development of ulceration in the colon along with a rise in histamine, MPO activity and oxidative stress. Treatment with Punica granatum extract and its ellagic acid rich fraction (100 mg/kg and 200 mg/kg p.o.) significantly attenuated DSS-induced colonic inflammation along with attenuation of histamine, MPO and oxidative stress. The antiulcerative effect of Punica granatum extract and its ellagic acid rich fraction were comparable to sulphasalazine (100 mg/kg, p.o.) and sodium cromoglycate (40 mg/kg i.p). It is concluded that Punica granatum has a potential for ameliorating DSS-induced colitis and its ellagic acid rich fraction may be responsible for this effect. Further, the antiulcerative effects may be attributed to mast cell stabilizing, antiinflammatory and antioxidant actions.
本研究旨在探讨石榴对葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎的改善作用。通过饮用 2%的 DSS 溶液来诱导溃疡性结肠炎，持续 7 天。通过宏观分析、组织病理学分析和疾病活动指数分析来评估溃疡的程度和严重程度。髓过氧化物酶（MPO），一种炎症的特异性标志物；组织胺，肥大细胞脱颗粒的标志物；超氧阴离子生成和脂质过氧化物。DSS 的给药导致结肠溃疡显著发展，同时组织胺、MPO 活性和氧化应激升高。用石榴提取物及其富含鞣花酸的部分（100mg/kg 和 200mg/kg，口服）治疗可显著减轻 DSS 诱导的结肠炎症，同时减轻组织胺、MPO 和氧化应激。石榴提取物及其富含鞣花酸的部分的抗溃疡作用与柳氮磺胺吡啶（100mg/kg，口服）和色甘酸钠（40mg/kg，腹腔注射）相当。结论是，石榴具有改善 DSS 诱导的结肠炎的潜力，其富含鞣花酸的部分可能是其发挥作用的原因。此外，抗溃疡作用可能归因于肥大细胞稳定、抗炎和抗氧化作用。
