Fletcher C Mark, Coyne Michael J, Villa Otto F, Chatzidaki-Livanis Maria, Comstock Laurie E
Channing Laboratory, Brigham & Women's Hospital, Harvard Medical School, 181 Longwood Avenue, Boston, MA 02115, USA.
Cell. 2009 Apr 17;137(2):321-31. doi: 10.1016/j.cell.2009.02.041.
The Bacteroides are a numerically dominant genus of the human intestinal microbiota. These organisms harbor a rare bacterial pathway for incorporation of exogenous fucose into capsular polysaccharides and glycoproteins. The infrequency of glycoprotein synthesis by bacteria prompted a more detailed analysis of this process. Here, we demonstrate that Bacteroides fragilis has a general O-glycosylation system. The proteins targeted for glycosylation include those predicted to be involved in protein folding, protein-protein interactions, peptide degradation as well as surface lipoproteins. Protein glycosylation is central to the physiology of B. fragilis and is necessary for the organism to competitively colonize the mammalian intestine. We provide evidence that general O-glycosylation systems are conserved among intestinal Bacteroides species and likely contribute to the predominance of Bacteroides in the human intestine.
拟杆菌属是人类肠道微生物群中数量占优势的属。这些微生物拥有一条罕见的细菌途径，可将外源岩藻糖掺入荚膜多糖和糖蛋白中。细菌进行糖蛋白合成的频率较低，这促使人们对这一过程进行更详细的分析。在此，我们证明脆弱拟杆菌具有一个通用的O-糖基化系统。被靶向进行糖基化的蛋白质包括那些预计参与蛋白质折叠、蛋白质-蛋白质相互作用、肽降解以及表面脂蛋白的蛋白质。蛋白质糖基化对于脆弱拟杆菌的生理学至关重要，并且是该生物体竞争性定殖于哺乳动物肠道所必需的。我们提供的证据表明，通用的O-糖基化系统在肠道拟杆菌物种中是保守的，并且可能有助于拟杆菌在人类肠道中的优势地位。
