Skov Olsen P, Nexø E, Poulsen S S, Hansen H F, Kirkegaard P
Regul Pept. 1984 Dec;10(1):37-45. doi: 10.1016/0167-0115(84)90051-x.
The origin of rat urinary epidermal growth factor (EGF) has been investigated. Unilateral nephrectomy decreased the concentration, total output of EGF and EGF/creatinine ratio by approximately 50%, while the output of creatinine was unchanged. Removal of the submandibular glands and duodenal Brunner's glands, organs known to produce EGF, had no influence on the output of EGF in urine. Renal clearance of EGF exceeded that of creatinine, and after bilateral nephrectomy or bilateral ligation of the ureters, the concentration of creatinine in serum increased, while the concentration of EGF was below the detection limit of the assay. Renal production of EGF was confirmed by immunohistochemistry demonstrating EGF immunoreactivity in the afferent arteriole of the juxtaglomerular apparatus. EGF in the submandibular glands and in urine was found to differ with chromatofocusing and reverse-phase HPLC. At isoelectric focusing the pI of submandibular EGF was 4.8 and 5.4 while that of urinary EGF was 5.3 and 6.4. In conclusion, this study demonstrates that urinary EGF mainly originates from the kidneys and is localized to the renal juxtaglomerular apparatus.
对大鼠尿表皮生长因子（EGF）的来源进行了研究。单侧肾切除使EGF的浓度、总输出量及EGF/肌酐比值降低了约50%，而肌酐的输出量未变。切除已知能产生EGF的下颌下腺和十二指肠布伦纳腺，对尿中EGF的输出量没有影响。EGF的肾清除率超过肌酐，双侧肾切除或双侧输尿管结扎后，血清中肌酐浓度升高，而EGF浓度低于检测限。免疫组织化学证实肾产生EGF，在肾小球旁器的入球小动脉中有EGF免疫反应性。通过色谱聚焦和反相高效液相色谱法发现下颌下腺和尿中的EGF有所不同。在等电聚焦时，下颌下EGF的pI为4.8和5.4，而尿EGF的pI为5.3和6.4。总之，本研究表明尿EGF主要来源于肾脏，且定位于肾肾小球旁器。
