Abergel C, Moulard M, Moreau H, Loret E, Cambillau C, Fontecilla-Camps J C
Laboratoire de Cristallographie et de Cristallisation des Macromolécules Biologiques, URA 1296-Centre National de la Recherche Scientifique, Faculté de Médecine Secteur-Nord, Marseille, France.
J Biol Chem. 1991 Oct 25;266(30):20131-8.
We have used the Incomplete Factorial Approach (Carter, C. W., and Carter, C. W., Jr. (1979) J. Biol. Chem. 254, 12219-12223) in conjunction with the program Cristal (Roussel, A., Serre, L., Frey M., and Fontecilla-Camps, J. (1990) J. Crystal Growth 106, 405-409) to crystallize six different proteins. We were able to obtain crystals and to identify the critical factors for crystallization for each of these six proteins. In some of the cases, we succeeded on the first try while using only minute amounts of protein. This study proves that the Incomplete Factorial Approach is a powerful tool in identifying the factors that need to be varied to achieve crystallization. Single crystals of adequate size were obtained for all the proteins reported here, although some did not diffract well enough to be studied by x-ray diffraction methods; the asymmetric units of these latter crystals contain a large metric units of these latter crystals contain a large number of molecules, which is most likely due to the presence of significant amounts of carbohydrate in the proteins.
我们采用不完全析因法（卡特，C.W.，以及卡特，C.W.，Jr.（1979年）《生物化学杂志》254卷，12219 - 12223页）并结合Cristal程序（鲁塞尔，A.，塞尔，L.，弗雷，M.，以及丰特西利亚 - 坎普斯，J.（1990年）《晶体生长杂志》106卷，405 - 409页）来结晶六种不同的蛋白质。我们成功获得了晶体，并确定了这六种蛋白质各自结晶的关键因素。在某些情况下，我们仅使用微量蛋白质就首次尝试成功。这项研究证明，不完全析因法是一种强大的工具，可用于确定为实现结晶而需要改变的因素。此处报道的所有蛋白质都获得了足够大小的单晶，尽管有些晶体的衍射效果不够好，无法用X射线衍射方法进行研究；后一种晶体的不对称单元包含大量分子，这很可能是由于蛋白质中存在大量碳水化合物所致。
