Hosaka Takeshi, Ohnishi-Kameyama Mayumi, Muramatsu Hideyuki, Murakami Kana, Tsurumi Yasuhisa, Kodani Shinya, Yoshida Mitsuru, Fujie Akihiko, Ochi Kozo
National Food Research Institute, Tsukuba, Ibaraki, Japan.
Nat Biotechnol. 2009 May;27(5):462-4. doi: 10.1038/nbt.1538. Epub 2009 Apr 26.
We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces actinomycetes species and 43% of Streptomyces species that do not produce antibacterials are activated to produce them. The antibacterial-producing mutants all carried mutations in RNA polymerase and/or the ribosomal protein S12.
我们发现，选择耐药性细菌突变体有助于发现抗菌化合物。从土壤中分离出的一种不产生抗菌物质的链霉菌属突变菌株合成了一类前所未知的抗菌物质，我们将其命名为哌啶霉素。总体而言，6% 的非链霉菌属放线菌物种和43% 不产生抗菌物质的链霉菌属物种被激活并开始产生抗菌物质。产生抗菌物质的突变体在RNA聚合酶和/或核糖体蛋白S12中均发生了突变。
